Generation of CHOPi-008-B, a euploid iPSC line from a patient with Trisomy 21 and a GATA1 mutation

Stem Cell Res. 2023 Oct:72:103198. doi: 10.1016/j.scr.2023.103198. Epub 2023 Sep 1.

Abstract

Transient myeloproliferative disorder (TMD) is a pre-leukemic condition that occurs only in neonates with Trisomy 21 (T21), and is attributed to a genetic interaction between the third copy of chromosome 21 (HSA21) and a mutation in the transcription factor GATA1 that results in a truncated protein (GATA1s). We generated a euploid iPSC line with a GATA1s mutation that is isogenic to a previously published pair of T21 lines with and without a GATA1 mutation. The line was characterized for pluripotency, differentiation potential, and genomic stability. This line is a valuable isogenic control for studying the T21 hematopoietic phenotype.

MeSH terms

  • Down Syndrome* / genetics
  • GATA1 Transcription Factor / genetics
  • Genomic Instability
  • Humans
  • Induced Pluripotent Stem Cells*
  • Infant, Newborn
  • Leukemia, Megakaryoblastic, Acute* / genetics
  • Mutation / genetics
  • Trisomy

Substances

  • GATA1 protein, human
  • GATA1 Transcription Factor