Methionine addition improves the acid tolerance of Lactiplantibacillus plantarum by altering cellular metabolic flux, energy distribution, lipids composition

Qiang Meng; Yueyao Li; Yuxin Yuan; Shaowen Wu; Kan Shi; Shuwen Liu

doi:10.1007/s44154-022-00072-z

Methionine addition improves the acid tolerance of Lactiplantibacillus plantarum by altering cellular metabolic flux, energy distribution, lipids composition

Stress Biol. 2022 Nov 14;2(1):48. doi: 10.1007/s44154-022-00072-z.

Authors

Qiang Meng^#¹, Yueyao Li^#¹, Yuxin Yuan¹, Shaowen Wu¹, Kan Shi^{2

3

4

5

6}, Shuwen Liu^{7

8

9

10

11}

Affiliations

¹ College of Enology, Northwest A&F University, Yangling, 712100, Shaanxi, China.
² College of Enology, Northwest A&F University, Yangling, 712100, Shaanxi, China. s.kan@nwafu.edu.cn.
³ Shaanxi Engineering Research Center for Viti-Viniculture, Yangling, 712100, Shaanxi, China. s.kan@nwafu.edu.cn.
⁴ Viti-viniculture Engineering Technology Center of State Forestry and Grassland Administration, Yangling, 712100, Shaanxi, China. s.kan@nwafu.edu.cn.
⁵ Heyang Experimental and Demonstrational Stations for Grape, Northwest A&F University, Heyang, 715300, Shaanxi, China. s.kan@nwafu.edu.cn.
⁶ Ningxia Helan Mountain's East Foothill Wine Experiment and Demonstration Station, Northwest A&F University, Yongning, 750104, Ningxia, China. s.kan@nwafu.edu.cn.
⁷ College of Enology, Northwest A&F University, Yangling, 712100, Shaanxi, China. liushuwen@nwsuaf.edu.cn.
⁸ Shaanxi Engineering Research Center for Viti-Viniculture, Yangling, 712100, Shaanxi, China. liushuwen@nwsuaf.edu.cn.
⁹ Viti-viniculture Engineering Technology Center of State Forestry and Grassland Administration, Yangling, 712100, Shaanxi, China. liushuwen@nwsuaf.edu.cn.
¹⁰ Heyang Experimental and Demonstrational Stations for Grape, Northwest A&F University, Heyang, 715300, Shaanxi, China. liushuwen@nwsuaf.edu.cn.
¹¹ Ningxia Helan Mountain's East Foothill Wine Experiment and Demonstration Station, Northwest A&F University, Yongning, 750104, Ningxia, China. liushuwen@nwsuaf.edu.cn.

^# Contributed equally.

Abstract

This paper reported a wine-derived lactic acid bacterium, Lactiplantibacillus plantarum XJ25, which exhibited higher cell viability under acid stress upon methionine supplementation. Cellular morphology and the composition of the cytomembrane phospholipids revealed a more solid membrane architecture presented in the acid-stressed cells treated with methionine supplementation. Transcriptional analysis showed L. plantarum XJ25 reduced methionine transport and homocysteine biosynthesis under acid stress. Subsequent overexpression assays proved that methionine supplementation could alleviate the cell toxicity from homocysteine accumulation under acid stress. Finally, L. plantarum XJ25 employed energy allocation strategy to response environmental changes by balancing the uptake methionine and adjusting saturated fatty acids (SFAs) in membrane. These data support a novel mechanism of acid resistance involving methionine utilization and cellular energy distribution in LAB and provide crucial theoretical clues for the mechanisms of acid resistance in other bacteria.

Keywords: Acid stress; Cell viability; Lactiplantibacillus plantarum; Membrane lipids; Methionine; Transcription level.

Abstract

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