Low-invasive neural recording in mouse models with diabetes via an ultrasmall needle-electrode

Rioki Sanda; Koji Yamashita; Hirohito Sawahata; Kensei Sakamoto; Shota Yamagiwa; Shohei Yokoyama; Rika Numano; Kowa Koida; Takeshi Kawano

doi:10.1016/j.bios.2023.115605

Low-invasive neural recording in mouse models with diabetes via an ultrasmall needle-electrode

Biosens Bioelectron. 2023 Nov 15:240:115605. doi: 10.1016/j.bios.2023.115605. Epub 2023 Aug 16.

Authors

Rioki Sanda¹, Koji Yamashita², Hirohito Sawahata³, Kensei Sakamoto¹, Shota Yamagiwa¹, Shohei Yokoyama⁴, Rika Numano⁵, Kowa Koida⁶, Takeshi Kawano⁷

Affiliations

¹ Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka Tempaku-cho, Toyohashi, 441-8580, Japan.
² Electronics-Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, 1-1 Hibarigaoka Tempaku-cho, Toyohashi, 441-8580, Japan.
³ National Institute of Technology, Ibaraki College, 866 Nakane, Hitachinaka, 312-8508, Japan.
⁴ TechnoPro, Inc., TechnoPro R&D Company, 6-10-1 Roppongi, Minato-ku, 106-6135, Japan.
⁵ Electronics-Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, 1-1 Hibarigaoka Tempaku-cho, Toyohashi, 441-8580, Japan; Department of Applied Chemistry and Life Science, Toyohashi University of Technology, 1-1 Hibarigaoka Tempaku-cho, Toyohashi, 441-8580, Japan.
⁶ Electronics-Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, 1-1 Hibarigaoka Tempaku-cho, Toyohashi, 441-8580, Japan; Department of Computer Science and Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka Tempaku-cho, Toyohashi, 441-8580, Japan.
⁷ Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka Tempaku-cho, Toyohashi, 441-8580, Japan; Electronics-Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, 1-1 Hibarigaoka Tempaku-cho, Toyohashi, 441-8580, Japan. Electronic address: kawano@ee.tut.ac.jp.

PMID: 37669586
DOI: 10.1016/j.bios.2023.115605

Abstract

Diabetes is known to cause a variety of complications, having a high correlation with Alzheimer's disease. Electrophysiological recording using a microscale needle electrode is a promising technology for the study, however, diabetic brain tissue is more difficult to record neuronal activities than normal tissue due to these complications including the development of cerebrovascular disease. Here we show an electrophysiological methodology for diabetic db/db mice (+Lepr^db/+Lepr^db) using a 4-μm-tip diameter needle-electrode device. The needle electrode minimized the tissue injury when compared to a typical larger metal electrode, as confirmed by bleeding during penetration. The proposed electrode device showed both acute and chronic in vivo recording capabilities for diabetic mice while reducing the glial cells' responses. Because of these device characteristics, the 4-μm-tip diameter needle-electrode will allow electrophysiological studies on diabetes models of not only mice, as proven in this study, but also other animals.

Keywords: Diabetes; Microelectrode; Neural recording.

MeSH terms

Alzheimer Disease*
Animals
Biosensing Techniques*
Diabetes Mellitus, Experimental*
Disease Models, Animal
Electrodes
Mice
Neuroglia