Developmental Toxicity Assessment Using Zebrafish-Based High-Throughput Screening

Subham Dasgupta; Michael T Simonich; Robyn L Tanguay

doi:10.1007/978-1-0716-3401-1_4

Developmental Toxicity Assessment Using Zebrafish-Based High-Throughput Screening

Methods Mol Biol. 2024:2707:71-82. doi: 10.1007/978-1-0716-3401-1_4.

Authors

Subham Dasgupta^{1

2}, Michael T Simonich¹, Robyn L Tanguay³

Affiliations

¹ Sinnhuber Aquatic Research Laboratory, Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, USA.
² Department of Biological Sciences, Clemson University, Clemson, SC, USA.
³ Sinnhuber Aquatic Research Laboratory, Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, USA. robyn.tanguay@oregonstate.edu.

PMID: 37668905
DOI: 10.1007/978-1-0716-3401-1_4

Abstract

Zebrafish-based high-throughput screening has been extensively used to study toxicological profiles of individual chemicals and mixtures, identify novel toxicants, and study modes of action to prioritize chemicals for further testing and policy decisions. Within this chapter, we describe a protocol for automated zebrafish developmental high-throughput screening in our laboratory, with emphasis on exposure setups, morphological and behavioral readouts, and quality control.

Keywords: Automation; Behavior; Chemicals; Development; Embryos; High-throughput screening; Morphology; Zebrafish.

MeSH terms

Animals
Hazardous Substances
High-Throughput Screening Assays*
Laboratories
Policy
Zebrafish*

Substances

Hazardous Substances

Grants and funding

P42 ES016465/ES/NIEHS NIH HHS/United States