Olea europaea L. Leaf Extract Alleviates Fibrosis Progression and Oxidative Stress Induced by Bleomycin on a Murine Model of Lung Fibrosis

Sana Bahri; Anouar Abidi; Afef Nahdi; Raed Abdennabi; Mona Mlika; Ridha Ben Ali; Saloua Jameleddine

doi:10.1177/15593258231200972

Olea europaea L. Leaf Extract Alleviates Fibrosis Progression and Oxidative Stress Induced by Bleomycin on a Murine Model of Lung Fibrosis

Dose Response. 2023 Sep 2;21(3):15593258231200972. doi: 10.1177/15593258231200972. eCollection 2023 Jul-Sep.

Authors

Sana Bahri^{1

2

3}, Anouar Abidi^{1

4}, Afef Nahdi⁵, Raed Abdennabi⁶, Mona Mlika⁷, Ridha Ben Ali⁸, Saloua Jameleddine^{1

2}

Affiliations

¹ Laboratory of Physiology, Faculty of Medicine of Tunis, University of Tunis El Manar, Tunis, Tunisia.
² Laboratory of Physiopathology, Food and Biomolecules (LR-17-ES-03), Technology Center of Sidi Thabet, University of Manouba, Tunis, Tunisia.
³ Laboratory of Quality Control, HERBES DE TUNISIE, Company AYACHI-Group, Mansoura, Siliana-Tunisia.
⁴ Unit of Functional Physiology and Valorization of Bio-Resources of the Higher Institute of Biotechnology of Béja, University of Jendouba, Jendouba, Tunisia.
⁵ Research Unit n° 17/ES/13, Faculty of Medicine, University of Tunis El Manar, Tunis, Tunisia.
⁶ Laboratory of Plant Biotechnology, Faculty of Science, University of Sfax, Sfax, Tunisia.
⁷ Laboratory of Anatomy and Pathology, Abderhaman Mami Hospital, Ariana, Tunisia.
⁸ Laboratory of Experimental Medicine, Faculty of Medicine of Tunis, University of Tunis El Manar, Tunis, Tunisia.

Abstract

In this study, we aim to investigate the effect of industrial Olea europaea L. leaf extract (OLE) against bleomycin (BLM)-induced pulmonary fibrosis (PF) in rats. Male Wistar rats were treated with a single intratracheal injection of BLM (4 mg/kg) and a daily intraperitoneal injection of OLE (10, 20, and 40 mg/kg) for 4 weeks. Results of HPLC and LC-MS analysis revealed a large amount of oleuropein (15.43%/DW) in OLE. BLM induced apparent damage of lung architecture with condensed collagen bundles, increased lipid peroxidation which has been deduced from malondialdehyde (MDA) levels: (.9 ± .13 vs .25 ± .12 nmol/mg protein) and hydroxyproline content (.601 ± .22 vs .154 ± .139 mg/g of lung tissue) and decreased catalase (CAT) (5.93.10^-5 ± 4.23.10^-5 vs 6.41.10^-4 ± 2.33.10^-4 μmol/min/mg protein) and superoxide dismutase (SOD) (28.73 ± 3.34 vs 50.13 ± 2.1 USOD/min/mg protein) levels compared to the control. OLE treatment (40 mg/kg) stabilized MDA content (.32 ± .15 and .27 ± .13 vs .9 ± .13 nmol/mg protein), normalized SOD (61.27 ± 13.37 vs 28.73 ± 3.34 USOD/min/mg protein), and CAT (5.2.10^-4 ±1.8.10^-4 vs 5.93.10^-5 ± 4.23.10^-5 μmol/min/mg protein) activities and counteracted collagen accumulation and hydroxyproline content (.222 ± .07 vs .601 ± .22 mg/g of lung tissue) in the lung parenchyma. Finally, OLE might have a potent protective effect against PF by regulating oxidative parameters and attenuating collagen deposition, due to the existence of large amount of bioactive phenolic molecules.

Keywords: bleomycin; hydroxyproline; inflammation; lung fibrosis; oleuropein; oxidative stress.