Exploring Biological Interactions: A New Pyrazoline as a Versatile Fluorescent Probe for Energy Transfer and Cell Staining Applications

Burcu Meryem Beşer; Berat Yildirim

doi:10.1002/open.202300092

Exploring Biological Interactions: A New Pyrazoline as a Versatile Fluorescent Probe for Energy Transfer and Cell Staining Applications

ChemistryOpen. 2023 Sep;12(9):e202300092. doi: 10.1002/open.202300092.

Authors

Burcu Meryem Beşer¹, Berat Yildirim¹

Affiliation

¹ Faculty of Arts and Sciences, Department of Chemistry, Erzincan Binali Yıldırım University, Erzincan, Türkiye.

Abstract

Fluorescent dyes are used in biological systems, because they are highly sensitive and selective. In this work, we investigated the fluorescent probe properties of 2-(5-(pyridin-2-yl)-1H-pyrazol-3-yl) phenol (PYDP) in two media [sodium dodecyl sulfate (SDS) and human serum albumin (HSA)]. Energy transfer parameters, photophysical and thermodynamic parameters of probe were determined. We investigated cytotoxicity of PYDP against colorectal adenocarcinoma cell lines (HT-29), breast cancer cell lines (MCF-7) and 3T3-L1 adipocytes (3T3 L1) cells. The cell staining property of PYDP was monitored using a confocal microscope. The results showed that PYDP binds to HSA, bindings are due to electrostatic/ionic interactions, and the binding process is spontaneous. PYDP was found to exhibit negligible cytotoxicity at high concentrations, and confocal microscope images showed that PYDP stained the cytoplasm of MCF-7 cells.

Keywords: Biophysics; FRET; cell imaging; cell staining; cytotoxicity; thermodynamic.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3-L1 Cells
Adenocarcinoma*
Animals
Energy Transfer
Fluorescent Dyes* / toxicity
HT29 Cells
Humans
Mice
Serum Albumin, Human

Substances

Fluorescent Dyes
Serum Albumin, Human