RNAi-based silencing of proteasome 20S subunit alpha 2 affected the survival and development of Henosepilachna vigintioctopunctata

Junna Liu; Mujuan Guo; Satyabrata Nanda; Zhaoyang Li; Xuguo Zhou; Youjun Zhang; Chunxiao Yang; Huipeng Pan

doi:10.1016/j.pestbp.2023.105547

RNAi-based silencing of proteasome 20S subunit alpha 2 affected the survival and development of Henosepilachna vigintioctopunctata

Pestic Biochem Physiol. 2023 Sep:195:105547. doi: 10.1016/j.pestbp.2023.105547. Epub 2023 Jul 23.

Authors

Junna Liu¹, Mujuan Guo¹, Satyabrata Nanda², Zhaoyang Li¹, Xuguo Zhou³, Youjun Zhang⁴, Chunxiao Yang⁵, Huipeng Pan⁶

Affiliations

¹ National Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
² MS Swaminathan School of Agriculture, Centurion University of Technology and Management, Paralakhemundi 761200, India.
³ Department of Entomology, University of Kentucky, Lexington 40546, USA.
⁴ Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
⁵ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China. Electronic address: yangchunxiao@scau.edu.cn.
⁶ National Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China. Electronic address: panhuipeng@scau.edu.cn.

PMID: 37666590
DOI: 10.1016/j.pestbp.2023.105547

Abstract

Henosepilachna vigintioctopunctata is a notorious pest of solanaceous plants in Asia, which is mainly managed by chemical pesticides. RNA interference (RNAi) technique is considered to be a promising and effective alternative for pest control. In this study, we selected the proteasome 20S subunit alpha 2 (Prosα2) gene, a cellular protein involved in many proteins regulatory processes, to explore the RNAi efficiency in H. vigintioctopunctata. The obtained results confirmed the significant lethal effects of HvProsα2 silencing on the H. vigintioctopunctata 1st instar larvae at concentrations of 100, 50, and 5 ng/μL. Ingestion of the bacterially expressed dsHvProsα2 caused high mortality in both larvae and adults. Moreover, silencing of HvProsα2 resulted in feeding disorders, growth delay, and abnormal intestinal development of the larvae. Overall, HvProsα2 acts as an important regulator for the growth and development of H. vigintioctopunctata, and can serve as a candidate target gene for the RNAi-based control of H. vigintioctopunctata.

Keywords: Biopesticide; Dietary RNAi; Feeding disorders; Henosepilachna vigintioctopunctata; Prosα2.

MeSH terms

Animals
Coleoptera*
Larva / genetics
Pesticides*
Proteasome Endopeptidase Complex
RNA Interference

Substances

Proteasome Endopeptidase Complex
Pesticides