Multi-residue determination of five quinoxalines and three their metabolites in poultry feathers and its application for depletions of olaquindox and quincetone in chickens

Song Zhanteng; Fan Xia; Zhu Jingrong; Xiao Zhiming; Li Yang; Wang Shi; Suo Decheng

doi:10.1016/j.jpba.2023.115684

Multi-residue determination of five quinoxalines and three their metabolites in poultry feathers and its application for depletions of olaquindox and quincetone in chickens

J Pharm Biomed Anal. 2023 Nov 30:236:115684. doi: 10.1016/j.jpba.2023.115684. Epub 2023 Aug 25.

Authors

Song Zhanteng¹, Fan Xia², Zhu Jingrong³, Xiao Zhiming², Li Yang², Wang Shi², Suo Decheng⁴

Affiliations

¹ Institute of Agricultural Quality Standards and Testing Technology, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang 830091, China; Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081, China.
² Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081, China.
³ Institute of Agricultural Quality Standards and Testing Technology, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang 830091, China. Electronic address: Zhujr2030@163.com.
⁴ Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081, China. Electronic address: suodecheng@caas.cn.

PMID: 37666119
DOI: 10.1016/j.jpba.2023.115684

Abstract

A method for the simultaneous determination of eight quinoxalines and their metabolites in poultry feathers was developed in this study using ultra-performance liquid chromatography combine with quadrupole linear ion trap electrostatic field orbit trap high resolution mass spectrometer. Specificity, matrix effect, linearity, trueness, repeatability, reproducibility, and stability using the Commission of the European Communities, Regulation (EU) 2021/808. The correlation coefficients were all greater than 0.99. The average recoveries of the eight compounds ranged from 76.1% to 112.8%, with coefficient of variation of less than 15%. The limits of detection and quantification of the method were 0.04-0.1 μg/kg and 0.12-0.3 μg/kg, respectively. The method has been successfully applied to depletions of olaquindox and quincetone in feathers of laying hens. Methylquinoxaline-2-carboxylic acid and quincetone are the main metabolites in olaquindox and quincetone in feather, respectively.

Keywords: Depletion; Feathers; Metabolites; Quinoxalines.