Effects of microplastics/nanoplastics on Vallisneria natans roots and sediment: Size effect, enzymology, and microbial communities

Abstract

Microplastics/nanoplastics (MNPs) pollution in different environmental media and its adverse effects on organisms have received increasing attention from researchers. This paper compares the effects of natural concentrations of three different sizes (20 nm, 200 nm, and 2 μm) of MNPs on Vallisneria natans and sediments. MNPs with smaller sizes adhere more readily to V. natans roots, further promoting root elongation. In addition, the larger the particle size of MNPs, the higher the reactive oxygen species level in the roots, and the malondialdehyde level increased accordingly. In the sediment, 20 nm, and 200 nm MNPs increased the activity of related enzymes, including acid phosphatase, urease, and nitrate reductase. In addition, the dehydrogenase content in the treated sediments increased, and the content changes were positively correlated with the size of MNPs. Changes in microorganisms were only observed on the root surface. The addition of MNPs reduced the abundance of Proteobacteria and increased the abundance of Chloroflexi. In addition, at the class level of species composition on the root surface, the abundance of Gammaproteobacteria under the 20 nm, 200 nm, and 2 μm MNP treatments decreased by 21.19%, 16.14%, and 17.03%, respectively, compared with the control group, while the abundance of Anaerolineae increased by 44.63%, 26.31%, and 62.52%, respectively. These findings enhance the understanding of the size effects of MNPs on the roots of submerged plants and sediment.

Keywords: Antioxidant system; Microbial community; Microplastics; Nanoplastics; Sediment enzyme; Vallisneria natans.