Expression data of FOS and JUN genes and FTIR spectra provide diagnosis of thyroid carcinoma

João Paulo da Silva Queiroz; Breno Pupin; Tanmoy Tapobrata Bhattacharjee; Miyuki Uno; Roger Chammas; Marco Aurélio Vamondes Kulcsar; Renata de Azevedo Canevari

doi:10.1016/j.saa.2023.123305

Expression data of FOS and JUN genes and FTIR spectra provide diagnosis of thyroid carcinoma

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jan 5:304:123305. doi: 10.1016/j.saa.2023.123305. Epub 2023 Aug 28.

Authors

João Paulo da Silva Queiroz¹, Breno Pupin¹, Tanmoy Tapobrata Bhattacharjee², Miyuki Uno³, Roger Chammas³, Marco Aurélio Vamondes Kulcsar⁴, Renata de Azevedo Canevari⁵

Affiliations

¹ Laboratório de Biologia Molecular do Câncer, Universidade do Vale do Paraíba, UNIVAP, Instituto de Pesquisa e Desenvolvimento, Avenida Shishima Hifumi 2911, Urbanova, São José dos Campos, 12244-000 São Paulo, SP, Brazil.
² Sir John Walsh Research Institute, University of Otago, 310 Great King St, North Dunedin, Dunedin 9016, New Zealand.
³ Centro de Investigação Translacional em Oncologia, Departamento de Radiologia e Oncologia, Instituto do Cancer do Estado de São Paulo (ICESP), Faculdade de Medicina da Universidade de São Paulo (FMUSP), Avenida Dr. Arnaldo 251, Cerqueira César, São Paulo 01246-000, São Paulo, Brazil.
⁴ Serviço de Cirurgia de cabeça e Pescoço, Instituto do Câncer do Estado de São Paulo - ICESP, Av. Doutor Arnaldo, 251, Cerqueira César, CEP 01246-000 São Paulo, SP, Brazil.
⁵ Laboratório de Biologia Molecular do Câncer, Universidade do Vale do Paraíba, UNIVAP, Instituto de Pesquisa e Desenvolvimento, Avenida Shishima Hifumi 2911, Urbanova, São José dos Campos, 12244-000 São Paulo, SP, Brazil. Electronic address: rcanevari@univap.br.

PMID: 37660502
DOI: 10.1016/j.saa.2023.123305

Abstract

We explore the feasibility of using FOS and JUN gene expression and ATR-FTIR for diagnosis of thyroid cancer. For the study, 38 samples (6 non-neoplastic (NN), 10 papillary thyroid carcinoma (PTC), 7 follicular thyroid carcinoma (FTC), and 15 benign tumors (BT) were subjected to RNA extraction followed by quantitative real time PCR (qRT-PCR) and 30 samples (5 NN, 9 PTC, 5 FTC, and 11 BT) were used for Attenuated Total Reflectance - Fourier Transform Infrared (ATR-FTIR) followed by multivariate analysis. Of the above, 20 samples were used for both gene expression and ATR-FTIR studies. We found FOS and JUN expression in malignant tumor samples to be significantly lower than NN and benign. ATR-FIR after multivariate analysis could identify the difficult to diagnose FTC with 93 % efficiency. Overall, results suggest the diagnostic potential of molecular biology techniques combined with ATR-FTIR spectroscopy in differentiated thyroid carcinomas (PTC and FTC) and BT.

Keywords: Diagnosis; FOS; FTIR; Gene expression; JUN; Thyroid cancer.