Vitrification induces a focused spindle pole in mouse MI oocytes

Yaoyao Guo; Haowei Sun; Hui Chen; Gongshe Yang; Jialun Wang; Zhengjun Qi; Weijun Pang; Guiyan Chu; Lei Gao

doi:10.1016/j.theriogenology.2023.08.020

Vitrification induces a focused spindle pole in mouse MI oocytes

Theriogenology. 2023 Nov:211:232-240. doi: 10.1016/j.theriogenology.2023.08.020. Epub 2023 Aug 26.

Authors

Yaoyao Guo¹, Haowei Sun², Hui Chen³, Gongshe Yang⁴, Jialun Wang⁵, Zhengjun Qi⁶, Weijun Pang⁷, Guiyan Chu⁸, Lei Gao⁹

Affiliations

¹ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: GYY19981111@163.com.
² Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: shwwj325@163.com.
³ Animal Husbandry Industry Test and Demonstration Center of Shaanxi Province, Jingyang, 713708, Shaanxi, China. Electronic address: chenhui089@163.com.
⁴ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: gsyang999@hotmail.com.
⁵ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: 1067875173@qq.com.
⁶ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: qizj2001@163.com.
⁷ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: pwj1226@nwafu.edu.cn.
⁸ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: guiyanchu@nwafu.edu.cn.
⁹ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: gaolei5414@nwafu.edu.cn.

PMID: 37660475
DOI: 10.1016/j.theriogenology.2023.08.020

Abstract

Immature oocyte (germinal vesicle stage, GV) vitrification can avoid a cycle of ovarian stimulation, which is friendly to patients with hormone-sensitive tumors. However, the in vitro maturation of vitrification-thawed GV oocyte usually results in aneuploidy, and the underlying mechanism remains unclear. Stable spindle poles are important for accurate chromosome segregation. Acentriolar microtubule-organizing centers (aMTOCs) undergo fragmentation and reaggregation to form spindle poles. Microtubule nucleation is facilitated via the perichromosome Ran after GVBD, which plays an important role in aMTOCs fragmentation. This study showed that vitrification may reduce microtubule density by decreasing perichromosomal Ran levels, which reduced the localization of pKIF11, thereby decreased the fragmentation of aMTOCs and formed a more focused spindle pole, ultimately resulted in aneuploidy. This study revealed the mechanism of abnormal spindle pole formation in vitrified oocytes and offered a theoretical support to further improve the quality of vitrified oocytes.

Keywords: Microtubules; Oocyte vitrification; Ran; Spindle pole; aMTOCs.

MeSH terms

Aneuploidy
Animals
Cell Cycle
Mice
Oocytes
Spindle Poles
Vitrification*