Injectable and photothermal antibacterial bacterial cellulose cryogel for rapid hemostasis and repair of irregular and deep skin wounds

Shujun Cao; Kun Zhang; Qiujing Li; Shukun Zhang; Jingdi Chen

doi:10.1016/j.carbpol.2023.121239

Injectable and photothermal antibacterial bacterial cellulose cryogel for rapid hemostasis and repair of irregular and deep skin wounds

Carbohydr Polym. 2023 Nov 15:320:121239. doi: 10.1016/j.carbpol.2023.121239. Epub 2023 Jul 27.

Authors

Shujun Cao¹, Kun Zhang¹, Qiujing Li², Shukun Zhang³, Jingdi Chen⁴

Affiliations

¹ Marine College, Shandong University, Weihai 264209, China.
² Weihai Municipal Hospital, Cheeloo College of Medicine, Shandong University, Weihai 264299, China.
³ Weihai Municipal Hospital, Cheeloo College of Medicine, Shandong University, Weihai 264299, China. Electronic address: zhangshukun0475@126.com.
⁴ Marine College, Shandong University, Weihai 264209, China. Electronic address: jdchen@sdu.edu.cn.

PMID: 37659822
DOI: 10.1016/j.carbpol.2023.121239

Abstract

For irregular and deep skin wounds, it's difficult for wound dressing to reach the injured site to achieve rapid hemostasis and provide wound protection. Bacterial cellulose (BC) has high strength and natural three-dimensional pore structure, which endows it shape recovery ability after absorbing blood when injected to the wound. Therefore, in the study, an injectable aldehyde bacterial cellulose/polydopamine (DBC/PDA) photothermal cryogel was prepared by oxidation polymerization method for hemostasis and repair of irregular and deep skin wounds. BC was oxidized by NaIO₄ to form DBC and dopamine (DA) was introduced into DBC by reacting with the aldehyde group in DBC through Schiff base reaction. Under oxidation effect of NaIO₄ and with freezing condition, water crystallization led to local aggregation of DA and DBC, and at the same time DA was oxidized to PDA and polymerized with DA on DBC. After the melting process, the porous cryogel was obtained. The introduction of PDA enhances the photothermal properties of DBC/PDA cryogel. DBC/PDA cryogel can kill most bacteria and provide wound protection under near-infrared light. In vitro and in vivo hemostatic tests show that the DBC/PDA cryogel can quickly absorb blood and stop bleeding. Combined with its good injectable, DBC/PDA cryogel can provide rapid hemostatic and protection in the face of irregular and deep skin wounds.

Keywords: Bacterial cellulose; Cryogel; Hemostasis; Near-infrared light; Wound healing.