Arabinoxylan source and xylanase specificity influence the production of oligosaccharides with prebiotic potential

Reskandi C Rudjito; Amparo Jiménez-Quero; Maria Del Carmen Casado Muñoz; Teun Kuil; Lisbeth Olsson; Mary Ann Stringer; Kristian Bertel Rømer Mørkeberg Krogh; Jens Eklöf; Francisco Vilaplana

doi:10.1016/j.carbpol.2023.121233

Arabinoxylan source and xylanase specificity influence the production of oligosaccharides with prebiotic potential

Carbohydr Polym. 2023 Nov 15:320:121233. doi: 10.1016/j.carbpol.2023.121233. Epub 2023 Jul 24.

Authors

Reskandi C Rudjito¹, Amparo Jiménez-Quero², Maria Del Carmen Casado Muñoz³, Teun Kuil⁴, Lisbeth Olsson⁵, Mary Ann Stringer⁶, Kristian Bertel Rømer Mørkeberg Krogh⁷, Jens Eklöf⁸, Francisco Vilaplana⁹

Affiliations

¹ Division of Glycoscience, Department of Chemistry, KTH Royal Institute of Technology, AlbaNova University Centre, SE-106 91 Stockholm, Sweden. Electronic address: rudjito@kth.se.
² Division of Glycoscience, Department of Chemistry, KTH Royal Institute of Technology, AlbaNova University Centre, SE-106 91 Stockholm, Sweden. Electronic address: amparojq@kth.se.
³ Division of Glycoscience, Department of Chemistry, KTH Royal Institute of Technology, AlbaNova University Centre, SE-106 91 Stockholm, Sweden. Electronic address: mdc2@kth.se.
⁴ Department of Industrial Biotechnology, KTH Royal Institute of Technology, AlbaNova University Centre, SE-106 91 Stockholm, Sweden. Electronic address: teun@kth.se.
⁵ Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 412 96 Gothenburg, Sweden; Wallenberg Wood Science Center, Chalmers University of Technology, Kemigården 4, 412 96 Gothenburg, Sweden. Electronic address: lisbeth.olsson@chalmers.se.
⁶ Novozymes A/S, Krogshøjvej 36, 2880 Bagsværd, Denmark. Electronic address: MSTR@novozymes.com.
⁷ Novozymes A/S, Krogshøjvej 36, 2880 Bagsværd, Denmark. Electronic address: KBK@novozymes.com.
⁸ Novozymes A/S, Krogshøjvej 36, 2880 Bagsværd, Denmark. Electronic address: JEEQ@novozymes.com.
⁹ Division of Glycoscience, Department of Chemistry, KTH Royal Institute of Technology, AlbaNova University Centre, SE-106 91 Stockholm, Sweden; Wallenberg Wood Science Centre, KTH Royal Institute of Technology, Teknikringen 56-58, SE-100 44 Stockholm, Sweden. Electronic address: franvila@kth.se.

PMID: 37659797
DOI: 10.1016/j.carbpol.2023.121233

Abstract

Cereal arabinoxylans (AXs) are complex polysaccharides in terms of their pattern of arabinose and ferulic acid substitutions, which influence their properties in structural and nutritional applications. We have evaluated the influence of the molecular structure of three AXs from wheat and rye with distinct substitutions on the activity of β-xylanases from different glycosyl hydrolase families (GH 5_34, 8, 10 and 11). The arabinose and ferulic acid substitutions influence the accessibility of the xylanases, resulting in specific profiles of arabinoxylan-oligosaccharides (AXOS). The GH10 xylanase from Aspergillus aculeatus (AcXyn10A) and GH11 from Thermomyces lanuginosus (TlXyn11) showed the highest activity, producing larger amounts of small oligosaccharides in shorter time. The GH8 xylanase from Bacillus sp. (BXyn8) produced linear xylooligosaccharides and was most restricted by arabinose substitution, whereas GH5_34 from Gonapodya prolifera (GpXyn5_34) required arabinose substitution and produced longer (A)XOS substituted on the reducing end. The complementary substrate specificity of BXyn8 and GpXyn5_34 revealed how arabinoses were distributed along the xylan backbones. This study demonstrates that AX source and xylanase specificity influence the production of oligosaccharides with specific structures, which in turn impacts the growth of specific bacteria (Bacteroides ovatus and Bifidobacterium adolescentis) and the production of beneficial metabolites (short-chain fatty acids).

Keywords: Arabinoxylan structure; Cereal arabinoxylans; Ferulic acid; Prebiotics; Xylanases.