Protective effect of juglone on electric field-induced apoptosis and inflammation in liver and kidney tissue in rats

Abstract

Electric field (EF) has been shown to cause tissue damage mainly through oxidative stress, inflammation, and apoptosis. Thus, juglone (5-hydroxy-1,4-naphthoquinone) (JUG), which has antioxidant and antiapoptotic properties, is thought to be effective against electric field-induced damage. We aimed to investigate whether 50 Hz alternating current (AC) triggers inflammation and apoptosis in rat liver and kidney tissues and evaluate the JUG supplement's estimated protective effect. Twenty-four adult male wistar albino rats were divided into control, EF and EF + JUG groups, each containing eight rats. The EF and EF + JUG groups were exposed to EF while no EF exposure and JUG were applied to the control group. At the end of the experiment, liver and kidney tissues were collected for histological (H&E, caspase-3 and TNF-α for immunohistochemical staining), and genetics (SOCS, caspase-3 and TNF-α, PCR analyses). After routine histological procedures, sections stained with H&E showed significant changes in liver and kidney tissues in the EF group compared to the control group (p < 0.05). Significant protective effects were observed in the building volumes and histopathology in the EF + JUG group (p < 0.05). Our gene expression results increased the expression of caspase-3 and TNF-α in the EF group (p < 0.001). Juglone increased SOCS expression (p < 0.001). These findings were consistent with the anti-apoptotic and anti-inflammatory effects of JUG treatment. We reasoned that exposure to EF damaged rat liver and kidney tissues and administration of JUG alleviated the complications caused by 50 Hz EF.

Keywords: Electric field; Inflammation; Juglone; Kidney; Liver.