Generating hematopoietic cells from human pluripotent stem cells: approaches, progress and challenges

Haiqiong Zheng; Yijin Chen; Qian Luo; Jie Zhang; Mengmeng Huang; Yulin Xu; Dawei Huo; Wei Shan; Ruxiu Tie; Meng Zhang; Pengxu Qian; He Huang

doi:10.1186/s13619-023-00175-6

Generating hematopoietic cells from human pluripotent stem cells: approaches, progress and challenges

Cell Regen. 2023 Sep 1;12(1):31. doi: 10.1186/s13619-023-00175-6.

Authors

Haiqiong Zheng^#^{1

2

3

4}, Yijin Chen^#^{1

2

3

4}, Qian Luo^#^{1

2

3

4}, Jie Zhang^{1

2

3

4}, Mengmeng Huang^{1

2

3

4}, Yulin Xu^{1

2

3

4}, Dawei Huo^{1

2

3

4}, Wei Shan^{1

2

3

4}, Ruxiu Tie^{1

2

3

4}, Meng Zhang^{5

6

7

8}, Pengxu Qian^{9

10

11

12}, He Huang^{13

14

15

16}

Affiliations

¹ Bone Marrow Transplantation Center, the First Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, 310012, China.
² Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou, 311121, China.
³ Institute of Hematology, Zhejiang University, Hangzhou, 310012, China.
⁴ Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy, Hangzhou, 310012, China.
⁵ Bone Marrow Transplantation Center, the First Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, 310012, China. zhangm1990@zju.edu.cn.
⁶ Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou, 311121, China. zhangm1990@zju.edu.cn.
⁷ Institute of Hematology, Zhejiang University, Hangzhou, 310012, China. zhangm1990@zju.edu.cn.
⁸ Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy, Hangzhou, 310012, China. zhangm1990@zju.edu.cn.
⁹ Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou, 311121, China. axu@zju.edu.cn.
¹⁰ Institute of Hematology, Zhejiang University, Hangzhou, 310012, China. axu@zju.edu.cn.
¹¹ Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy, Hangzhou, 310012, China. axu@zju.edu.cn.
¹² Center for Stem Cell and Regenerative Medicine and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China. axu@zju.edu.cn.
¹³ Bone Marrow Transplantation Center, the First Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, 310012, China. huanghe@zju.edu.cn.
¹⁴ Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou, 311121, China. huanghe@zju.edu.cn.
¹⁵ Institute of Hematology, Zhejiang University, Hangzhou, 310012, China. huanghe@zju.edu.cn.
¹⁶ Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy, Hangzhou, 310012, China. huanghe@zju.edu.cn.

^# Contributed equally.

Abstract

Human pluripotent stem cells (hPSCs) have been suggested as a potential source for the production of blood cells for clinical application. In two decades, almost all types of blood cells can be successfully generated from hPSCs through various differentiated strategies. Meanwhile, with a deeper understanding of hematopoiesis, higher efficiency of generating progenitors and precursors of blood cells from hPSCs is achieved. However, how to generate large-scale mature functional cells from hPSCs for clinical use is still difficult. In this review, we summarized recent approaches that generated both hematopoietic stem cells and mature lineage cells from hPSCs, and remarked their efficiency and mechanisms in producing mature functional cells. We also discussed the major challenges in hPSC-derived products of blood cells and provided some potential solutions. Our review summarized efficient, simple, and defined methodologies for developing good manufacturing practice standards for hPSC-derived blood cells, which will facilitate the translation of these products into the clinic.

Keywords: Blood cells; Hematopoietic differentiation; Hematopoietic stem cells; Human pluripotent stem cells.

Publication types

Review

Abstract

Publication types

Grants and funding