A wide-range UAC sensor for the classification of hyperuricemia in spot samples

Anran Zheng; Zhen Guo; Chao Li; Zhiqi Zhang; Chuanyu Li; Jia Yao; Xin Wang; Jinze Li; Shasha Zhao; Weiguo Wang; Wei Zhang; Lianqun Zhou

doi:10.1016/j.talanta.2023.125102

A wide-range UAC sensor for the classification of hyperuricemia in spot samples

Talanta. 2024 Jan 1;266(Pt 2):125102. doi: 10.1016/j.talanta.2023.125102. Epub 2023 Aug 22.

Authors

Anran Zheng¹, Zhen Guo², Chao Li³, Zhiqi Zhang¹, Chuanyu Li², Jia Yao¹, Xin Wang⁴, Jinze Li¹, Shasha Zhao³, Weiguo Wang⁵, Wei Zhang⁶, Lianqun Zhou⁷

Affiliations

¹ CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, China; School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230026, China.
² CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, China; School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230026, China; Suzhou CASENS Co., Ltd, Suzhou, 215163, China.
³ CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, China.
⁴ Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China.
⁵ Suzhou Hospital, Affiliated Hospital of Medical School, Nanjing University, Suzhou, 215153, Jiangsu Province, China. Electronic address: shuodanwang@ln.hk.
⁶ CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, China; School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230026, China. Electronic address: zhangw@sibet.ac.cn.
⁷ CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, China; School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230026, China; Suzhou CASENS Co., Ltd, Suzhou, 215163, China. Electronic address: zhoulq@sibet.ac.cn.

PMID: 37651905
DOI: 10.1016/j.talanta.2023.125102

Abstract

Hyperuricemia (HUA) has received wide attention as an independent risk factor for various chronic diseases. HUA is usually asymptomatic, and the related damage can be reduced by effective classification and treatment according to uric acid clearance (UAC). UAC is a calculated ratio based on the uric acid level in blood and urine. This important method is not universally used due to the inconvenience of collecting 24-h urine samples in the clinic, and most sensors are limited by the need for wide ranges and for two testing samples. In this study, a pH-sensitive urate oxidase-modified electrochemical sensor with filter membrane was proposed to calculate UAC by detecting uric acid in blood and urine. The results demonstrated that the sensor had high selectivity for uric acid with a detection limit of 0.25 μM in 5 μL spot sample, the wide linear range was 2.5-7000 μM, and the impact of the sample pH was calibrated. The linear correlation of the measurement results between the UAC sensor and clinical instrument was higher than 0.980 for 87 patients. The change in UAC in spot urine may reflect alteration in body-transport mechanisms. Thus, the UAC sensor may open a new window for the management of HUA and broaden its application in point-of-care testing.

Keywords: Electrochemical sensor; Hyperuricemia; Point-of-care testing; Spot urine; Uric acid clearance.

MeSH terms

Body Fluids*
Humans
Hyperuricemia* / diagnosis
Kinetics
Point-of-Care Testing
Uric Acid

Substances

Uric Acid