Given the worldwide increase in diabetes, there is an urgent need for glucose sensors that can achieve the on-body detection of glucose concentration. With the development of nanomaterials and flexible electronics, wearable electrochemical enzyme-free glucose biosensors that can conveniently, continuously and stably monitor the glucose concentrations of diabetes patients without invasion and risk of infection are coming into focus. However, despite the enormous efforts toward wearable electrochemical enzyme-free glucose sensors, there have been limited achievements in developing a stretchable and breathable glucose sensor with high sensitivity, low detection limit, and excellent catalytic activity towards glucose oxidation in neutral media, to meet the need for continuous wearable glucose monitoring in scenarios such as the on-body detection of glucose in human sweat. Herein, we demonstrate a novel electrochemical enzyme-free glucose-sensing patch on the foundation of electrospun polyurethane (PU) fibrous mats to address some of the aforementioned challenges. The sensing patch was fabricated through a facile technology of electrospinning, followed by magnetron sputtering of gold (Au) to enable high conductivity. After that, ultrasonic-assisted electrodeposition was utilized to in situ introduce well-dispersed platinum nano pine needles along each fiber. Due to the good stretchability of PU materials, porous structure, and large specific surface area of electrochemical sites, the glucose-sensing patch promises merits such as good stretchability (performs well under 10% strain), high sensitivity (203.13 μA mM-1 cm-1), prominently low detection limit (14.77 μM), excellent selectivity, and efficient vapor permeability. Notably, the advanced hierarchical nanostructures with excellent catalytic activity towards glucose oxidation could be capable of detecting glucose in neutral conditions (pH = 7.4) without the assistance of enzymes. Given the facile fabrication methods and the integrated superior performances, this enzyme-free glucose-sensing patch could play a vital role in wearable glucose sensors.