CO2-responsive deep eutectic solvents for the enhanced extraction of hesperidin from Fertile orange peel

Shanshan Wang; Tian Lei; Leilei Liu; Zhijian Tan

doi:10.1016/j.foodchem.2023.137255

CO₂-responsive deep eutectic solvents for the enhanced extraction of hesperidin from Fertile orange peel

Food Chem. 2024 Jan 30:432:137255. doi: 10.1016/j.foodchem.2023.137255. Epub 2023 Aug 22.

Authors

Shanshan Wang¹, Tian Lei², Leilei Liu³, Zhijian Tan⁴

Affiliations

¹ Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, National and Local United Engineering Laboratory of Integrative Utilization of Eucommia Ulmoides, Jishou University, Zhangjiajie 427000, China.
² College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China.
³ Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, National and Local United Engineering Laboratory of Integrative Utilization of Eucommia Ulmoides, Jishou University, Zhangjiajie 427000, China; College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China. Electronic address: leileiliu2019@jsu.edu.cn.
⁴ Institute of Bast Fiber Crops & Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China. Electronic address: tanzhijian@caas.cn.

PMID: 37643516
DOI: 10.1016/j.foodchem.2023.137255

Abstract

Hesperidin, also known as vitamin P, is widely studied for its distinctive potential in food and pharmaceutical industries. This work focuses on the research of CO₂-responsive deep eutectic solvents (DESs) used as recyclable extractants for efficient extraction of hesperidin from Fertile orange peel. Reversible phase transformation of DES solution was achieved by bubbling CO₂/N₂. The maximum extraction yield of 22.39 mg/g was obtained for hesperidin under the following conditions: DES-6 (triethanolamine: 4-methoxyphenol = 1:1) used as extractant, water content of 35 wt%, solid-liquid ratio of 1:60 g/mL, extraction temperature of 25 °C, and ultrasonic time of 25 min, which was much higher than that extracted by methanol. Hesperidin of 74.38% was recovered in top phase after CO₂ triggering. Density function theory (DFT) results indicated that hydrogen and π-π bonds were the main factors affecting hesperidin extraction. This study provides a new idea for extracting natural bioactive compounds using recyclable extractants.

Keywords: Bioactive compounds; CO(2)-responsive deep eutectic solvents; Extraction; Hesperidin; Recyclable extractants.

MeSH terms

Carbon Dioxide
Citrus sinensis*
Deep Eutectic Solvents
Flavonoids
Hesperidin*

Substances

Hesperidin
Carbon Dioxide
Deep Eutectic Solvents
Flavonoids