Knockdown LIMP2 inhibits colorectal cancer cells migration, invasion, and metastasis

Yu Tian; Liumei Liang; Junxiong Chen; Jiaqi Liu; Yixi Su; Mengchen Shi; Weiqian Li; Jingdan Zhang; Yanchun Feng; Lingyuan He; Huanliang Liu; Xiangling Yang

doi:10.1016/j.yexcr.2023.113757

Knockdown LIMP2 inhibits colorectal cancer cells migration, invasion, and metastasis

Exp Cell Res. 2023 Oct 1;431(1):113757. doi: 10.1016/j.yexcr.2023.113757. Epub 2023 Aug 26.

Authors

Affiliations

¹ Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510655, China; Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510655, China.
² Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510655, China; Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510655, China. Electronic address: liuhuanl@mail.sysu.edu.cn.
³ Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510655, China; Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510655, China. Electronic address: yangxl28@mail.sysu.edu.cn.

PMID: 37640260
DOI: 10.1016/j.yexcr.2023.113757

Abstract

Colorectal cancer (CRC) is a common malignancy worldwide nowadays and liver metastasis is the primary cause of death in patients with CRC. Although lysosomal integral membrane protein 2 (LIMP2) has been reported to play important roles in gastric cancer and prostate cancer, its role in CRC remains unclear. The aim of this study was to investigate the function of LIMP2 in CRC invasion and migration, along with the potential underlying molecular mechanisms. We found that LIMP2 levels were higher in CRC tissues compared to adjacent normal tissues. Kaplan-Meier survival analysis showed that high expression of LIMP2 was associated with worse prognosis in CRC patients. Knockdown of LIMP2 significantly inhibited invasion, migration, and wound healing abilities of CRC cells in vitro, and inhibited CRC liver metastasis in vivo. Additionally, LIMP2 knockdown inhibited autophagy in CRC. Therefore, LIMP2 plays an important role in CRC progression. High expression of LIMP2 was associated with worse prognosis in CRC patients. Knockdown LIMP2 can effectively inhibit CRC cell migration and invasion in vitro and prevent liver metastasis in vivo. These findings suggest that LIMP2 may serve as an independent prognostic factor and potential therapeutic target for CRC.

Keywords: Autophagy; Colorectal cancer; LIMP2; Migration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Movement / genetics
Colorectal Neoplasms* / genetics
Humans
Liver Neoplasms* / genetics
Lysosomal Membrane Proteins
Male
Prostatic Neoplasms*

Substances

Lysosomal Membrane Proteins