Isolation and Flow Cytometry Analysis of Macrophages from the Kidney

Sarah J Miller; Alex Yashchenko; Kurt A Zimmerman

doi:10.1007/978-1-0716-3437-0_12

Isolation and Flow Cytometry Analysis of Macrophages from the Kidney

Methods Mol Biol. 2024:2713:171-181. doi: 10.1007/978-1-0716-3437-0_12.

Authors

Sarah J Miller¹, Alex Yashchenko¹, Kurt A Zimmerman²

Affiliations

¹ Department of Internal Medicine, Division of Nephrology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
² Department of Internal Medicine, Division of Nephrology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA. kurt-zimmerman@ouhsc.edu.

PMID: 37639123
DOI: 10.1007/978-1-0716-3437-0_12

Abstract

Renal macrophages help maintain homeostasis, participate in tissue injury and repair, and play a vital role in immune surveillance [1-3]. Kidney macrophages can be broken down into two subsets, infiltrating macrophages, which can be further broken down into Ly6C^hi and Ly6C^lo cells, and kidney resident macrophages. While recent studies have shed light on the differing origins and niches of these cells, a more thorough understanding of kidney macrophage populations and how they may respond to various conditions is needed. This protocol describes how to efficiently isolate murine kidney macrophage populations for flow cytometry analysis.

Keywords: Flow cytometry; Infiltrating macrophage; Kidney; Kidney resident macrophages; Ly6Chi monocyte; Ly6Clo monocyte; Monocyte.

MeSH terms

Animals
Flow Cytometry
Homeostasis
Immunologic Surveillance*
Kidney*
Macrophages
Mice