Tumor-targeted bioactive nanoprobes visualizing of hydrogen peroxide for forecasting chemotherapy-exacerbated malignant prognosis

Fan Zhang; Yong Jia; Fangman Chen; Yawei Zhao; Li Li; Zhimin Chang

doi:10.3389/fbioe.2023.1226680

Tumor-targeted bioactive nanoprobes visualizing of hydrogen peroxide for forecasting chemotherapy-exacerbated malignant prognosis

Front Bioeng Biotechnol. 2023 Aug 11:11:1226680. doi: 10.3389/fbioe.2023.1226680. eCollection 2023.

Authors

Fan Zhang^{1

2}, Yong Jia³, Fangman Chen¹, Yawei Zhao³, Li Li^{1

2}, Zhimin Chang^{1

2}

Affiliations

¹ CAS Key Laboratory of Bio Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences, Suzhou, China.
² Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China.
³ School of Nursing, Jilin University, Changchun, Jilin, China.

Abstract

Introduction: Fluorescent visualization of hydrogen peroxide in the tumor microenvironment (TME) is conducive to predicting malignant prognosis after chemotherapy. Two photon microscopy has been employed for in vivo hydrogen peroxide detection owing to its advantages of deep penetration and low phototoxicity. Methods: In this study, a two-photon fluorescent probe (TPFP) was protected by mesoporous silica nanoparticles (MSNs) and masked by cloaking the cancer cell membranes (CM), forming a tumor-targeted bioactive nanoprobe, termed MSN@TPFP@CM. Results: This multifunctional nanoprobe allowed for the effective and selective detection of excessive hydrogen peroxide production in chemotherapeutic Etoposide (VP-16)-challenged tumor cells using two-photon microscopy. After specific accumulation in tumors, VP-16-MSN@TPFP@CM monitored tumor-specific hydrogen peroxide levels and revealed a positive correlation between oxidative stress in the TME and chemotherapy-exacerbated malignant prognosis. Discussion: Given the recent translation of fluorescent imaging into early clinical trials and the high biocompatibility of bioactive nanoprobes, our approach may pave the way for specific imaging of oxidative stress in solid tumors after treatment and provide a promising technology for malignant prognosis predictions.

Keywords: bioactive; cancer; chemotherapy; hydrogen peroxide; nanoprobe.

Grants and funding

This research was funded by the National Key R&D Program of China (No. 2021YFB3602200), the National Natural Science Foundation of China (No. 82172077, 22201298, 62027825, and 82071066), Natural Science Foundation of Shandong Province, China (No. ZR2022QB243), China Postdoctoral Science Foundation funded project (2021M702414), Jilin Scientific and Technological Development Program (20210508003RQ), the Innovation and Entrepreneurship Team of Jiangsu Province (JSSCTD202145), Cross disciplinary Research Fund of Shanghai Ninth people’s Hospital, Shanghai JiaoTong university School of Medicine (JYJC202010).