A potential hybrid nanocomposite of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and fullerene for bone tissue regeneration and sustained drug release against bone infections

Maria Eduarda Araújo Ribeiro; Noemi Raquel Checca Huaman; Márcio Manhães Folly; Jose Gregório Cabrera Gomez; Rubén J Sánchez Rodríguez

doi:10.1016/j.ijbiomac.2023.126531

A potential hybrid nanocomposite of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and fullerene for bone tissue regeneration and sustained drug release against bone infections

Int J Biol Macromol. 2023 Aug 25:251:126531. doi: 10.1016/j.ijbiomac.2023.126531. Online ahead of print.

Authors

Maria Eduarda Araújo Ribeiro¹, Noemi Raquel Checca Huaman², Márcio Manhães Folly³, Jose Gregório Cabrera Gomez⁴, Rubén J Sánchez Rodríguez⁵

Affiliations

¹ Advanced Materials Laboratory - LAMAV, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, Parque Califórnia, 28015-620 Campos dos Goytacazes, RJ, Brazil. Electronic address: 202111220016@pq.uenf.br.
² Centro Brasileiro de Pesquisas Físicas, R. Dr. Xavier Sigaud 150, 22290-180 Rio de Janeiro, RJ, Brazil.
³ Animal Health Laboratory, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, RJ, Brazil.
⁴ University of São Paulo, Av. Prof. Almeida Prado 1280, Butantâ, 05508-900 São Paulo, SP, Brazil.
⁵ Advanced Materials Laboratory - LAMAV, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, Parque Califórnia, 28015-620 Campos dos Goytacazes, RJ, Brazil.

PMID: 37634778
DOI: 10.1016/j.ijbiomac.2023.126531

Abstract

Developing a multifunctional biomaterial for bone filling and local antibiotic therapy is a complex challenge for bone tissue engineering. Hybrid nanocomposites of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV) with nanohydroxyapatite (nHA), fullerene (C₆₀), and vancomycin (VC) were produced by injection. Fullerene was successfully impregnated with VC, as seen in FTIR. The crystallinity degree of PHBHV was slightly reduced in the presence of C₆₀ and VC (64.3 versus 60.8 %), due to the plasticizing effect of these particles. It also resulted in a decrease in the glass transition temperature (T_g), observed by differential scanning calorimetry (DSC). Dense PHBHV/nHA/C₆₀/VC had a flexural elastic modulus 29 % higher than PHBHV, as a result of the good interface between PHBHV, C_60, and nHA - particles of high elastic modulus. Dense disks released 25.03 ± 4.27 % of VC for 14 days, which demonstrated its potential to be an alternative treatment to bone infections. Porous scaffolds of PHBHV/nHA/C₆₀/VC were 3D printed with a porosity of 50 % and porous size of 467 ± 70 μm, and had compression elastic modulus of 0.022 GPa, being a promising material to trabecular bone replacement. The plasticizing effect of C₆₀ improved the printability of the material. The hybrid nanocomposite was non-cytotoxic and showed good ability in adhering macrophage cells.

Keywords: Bone filling; Drug release; Fullerene; Hybrid nanocomposite; PHBHV.