Incorporation of zinc sulfide nanoparticles, Acinetobacter pittii and Bacillus velezensis to improve tomato plant growth, biochemical attributes and resistance against Rhizoctoniasolani

Abstract

Green nanobiotechnology and beneficial bacterial strains as biofertilizers are crucial in agriculture to achieve food security. Both these strategies have been individually studied in improving plant resistance against phytopathogens along with enhancing plant productivity. Therefore, objective of this study was to explore the eco-friendly and cost-effective approach of utilizing plant growth promoting and disease suppressing bacterial strains and nanoparticles, individually as well as in combination, as bio-stimulants to improve plant growth, antioxidant defense system, nutrition and yield of tomato. A pot experiment was conducted to investigate the zinc sulfide nanoparticles (ZnS NPs) synthesized by using Jacaranda mimosifolia flower extracts (JFE), Acinetobacter pittii and Bacillus velezensis either individually or in combinations to check their potential against Rhizoctonia solani in tomato to suppress root rot infection and improve growth and yield. Among all the combinations the JFE-ZnS NPs + B. velezensis compared to untreated infected plants showed minimum disease incidence and maximum significant protection (66%) against R. solani instigated root rot that was followed by JFE-ZnS NPs + A. pittii and individual application of JFE-ZnS NPs by 58%. The same treatment showed maximum significant increase in plant fresh and dry biomass. B. velezensis significantly increased the photosynthetic pigments when applied individually. However, JFE-ZnS NPs alone and in mixed treatments with B. velezensis efficiently improved total soluble protein, sugar and phenolic contents. The same interactive application of JFE-ZnS NPs + B. velezensis improved the tomato plant nutrition (silicon (Si), magnesium (Mg), calcium (Ca) and potassium (K)) and redox quenching status by improving the activity of antioxidant defense enzymes. Overall, the interactive use of JFE-ZnS NPs with A. pittii and B. velezensis very appropriately prepared the host plant to fight against the negative effects of root rot pathogen in tomato. Advancements in interactively investigating the nanoparticles with beneficial plant growth promoting bacterial strains importantly can contribute in resolving the challenges of food security. According to our information, this is a pioneer report for implying JFE-ZnS NPs in synergism with A. pittii and B. velezensis to hinder the root rot in tomatoes.

Keywords: Biocontrol; Defense enzymes; Induced resistance; Mineral nutrition; Nanotechnology; Tomato; Yield.