Combine with RNA-seq Reveals the Effect of Melatonin in the Synthesis of Melanin in Primary Melanocytes of Silky Fowls Black-Bone Chicken

Ting Yang; Lingling Qiu; Shihao Chen; Zhixiu Wang; Yong Jiang; Hao Bai; Yulin Bi; Guobin Chang

doi:10.3390/genes14081648

Combine with RNA-seq Reveals the Effect of Melatonin in the Synthesis of Melanin in Primary Melanocytes of Silky Fowls Black-Bone Chicken

Genes (Basel). 2023 Aug 18;14(8):1648. doi: 10.3390/genes14081648.

Authors

Ting Yang¹, Lingling Qiu¹, Shihao Chen², Zhixiu Wang¹, Yong Jiang¹, Hao Bai³, Yulin Bi¹, Guobin Chang¹

Affiliations

¹ Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
² Institute of Epigenetics and Epigenomics, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
³ Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China.

Abstract

(1) Background: It was found that the melanin of black-bone chicken has various effects such as scavenging DPPH free radicals and anti-oxidation, and the synthesis of melanin is affected by various factors including hormones. In addition, several studies have found that melatonin affects the melanoma cell synthesis of melanin, which has not been reported in chicken primary melanocytes; so, relevant studies were conducted. (2) Methods: In this study, chicken primary melanocytes were isolated and characterized, and then melanocytes were treated with different concentrations of melatonin to investigate the effects of melatonin on melanin synthesis in chicken melanocytes in terms of melanin synthesis-related genes, melanin content, and tyrosinase activity, and combined with RNA seq to detect the change in gene expression level of chicken melanocytes after melatonin treatment. (3) Results: We isolated and characterized primary melanocytes, and indirect immunofluorescence assay results showed positive melanocyte marker genes. RT-qPCR results showed that melatonin decreased the expression of melanin synthesis-related genes. In addition, melatonin reduced the melanin content and decreased the tyrosinase activity of melanocytes in the treated group. A total of 1703 differentially expressed genes were screened by RNA-seq, and in addition, in the KEGG results, the signaling pathway associated with melanin synthesis, and the mTOR signaling pathway were enriched. (4) Conclusions: Melatonin could decrease the synthesis of melanin in chicken primary melanocytes.

Keywords: chicken; melanin synthesis; melanocytes; melatonin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chickens / genetics
Melanins* / genetics
Melanocytes
Melatonin* / pharmacology
Monophenol Monooxygenase / genetics
RNA-Seq
Silk

Substances

Melanins
Melatonin
Monophenol Monooxygenase
Silk

Grants and funding

This work was supported by the earmarked fund for CARS (CARS-41), the Postgraduate Research & Practice Innovation Program of Jiangsu Province (grant number KYCX23_3595), and the Jiangsu Key Research and Development Program (BE2022341).