Tracing the pathways by which flood duration impacts soil bacteria through soil properties and water-extractable dissolved organic matter: A soil column experiment

Abstract

Soil microbial communities control biogeochemical processes, nutrient cycling, and organic carbon storage and release in wetlands, which are influenced by flooding. To predict soil nutrient function in wetland ecosystems, understanding the effect of flooding on soil biogeochemical cycling and energy flux, including soil properties, dissolved organic matter (DOM), and microbial communities is essential. This study investigated how different flood durations (1, 3, 8, 16, and 30 d) affect the interactions between physicochemical properties and bacterial communities in a river wetland. The DOM composition was measured using ultraviolet/visible spectrophotometry coupled with fluorescence spectroscopy, and the bacterial communities were identified using 16S rRNA sequencing. Simpson's diversity index varied from 0.92 to 0.94, indicating high bacterial diversity throughout the treatments; the highest and lowest bacterial diversities were found at 1 and 8 flooding days, respectively. The abundance of Desulturomonadales, Clostridiales, Bacteroidales, and Gaiellales was positively correlated with pH, electrical conductivity, water-extractable dissolved organic carbon (WEOC), and water-extractable total dissolved nitrogen (TDN) but negatively correlated with dissolved oxygen (DO) and soil organic matter (SOM), suggesting complex interactions among these factors in response to flooding. Structural equation model revealed that flooding directly increased TDN but indirectly increased WEOC through increasing soil pH; and directly decreased DO and SOM, leading to decreases in total protein-like fraction. Three significant pathways were identified, showing the impacts of flooding on bacterial diversity: (1) flood duration decreased DO, resulting in decreased bacterial diversity; (2) flood duration decreased SOM, leading to increased bacterial diversity; and (3) flood duration decreased DO and SOM, leading to increased bacterial diversity via decreased total protein-like fraction. This study indicated that prolonged flooding has both positive and negative impacts on bacterial diversity, depending on environmental factors. It highlights the importance of flooding in shaping soil bacterial communities, with implications for nutrient cycling and carbon storage in wetlands.

Keywords: Dissolved organic matter; Humic-like; Hydrologic changes; Protein-like; Soil bacterial communities.