Alternaria Mycotoxins Analysis and Exposure Investigation in Ruminant Feeds

Xin Mao; Wanzhao Chen; Huimin Wu; Ying Shao; Ya'ning Zhu; Qingyong Guo; Yanshen Li; Lining Xia

doi:10.3390/toxins15080495

Alternaria Mycotoxins Analysis and Exposure Investigation in Ruminant Feeds

Toxins (Basel). 2023 Aug 4;15(8):495. doi: 10.3390/toxins15080495.

Authors

Xin Mao¹, Wanzhao Chen¹, Huimin Wu¹, Ying Shao², Ya'ning Zhu², Qingyong Guo¹, Yanshen Li², Lining Xia¹

Affiliations

¹ Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animals, College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China.
² College of Life Science, Yantai University, Yantai 264000, China.

Abstract

Alternaria mycotoxins are a class of important, agriculture-related hazardous materials, and their contamination in ruminant feeds and products might bring severe toxic effects to animals and even human beings. To control these hazardous compounds, a reliable and sensitive LC-MS/MS (liquid chromatography-tandem mass spectrometry) method was established for simultaneous determination of six target Alternaria mycotoxins in ruminant feeds, including ALT (Altenuene), AME (Alternariol Monomethyl Ether), AOH (Alternariol), ATX-Ι (Altertoxins I), TeA (Tenuazonic Acid), and TEN (Tentoxin). This developed analytical method was used for the determination of the presence of these substances in cattle and sheep feeds in Xinjiang Province, China. The results revealed that Alternaria mycotoxins are ubiquitously detected in feed samples. Especially, AME, AOH, TeA, and TEN are the most frequently found mycotoxins with a positive rate over 40% and a concentration range of 4~551 µg/kg. The proposed method could be applied for exposure investigation of Alternaria mycotoxins in ruminant feeds and for the reduction in the health risk to animals and even consumers.

Keywords: Alternaria mycotoxin; LC-MS/MS; analysis; exposure investigation; ruminant feeds.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alternaria
Animals
Cattle
Chromatography, Liquid
Humans
Mycotoxins* / toxicity
Ruminants
Sheep
Tandem Mass Spectrometry

Substances

Mycotoxins

Grants and funding

This research was funded by the National Key Research and Development Program of China (No. 2021YFD1600702-3), Regional Fund of the National Natural Science Foundation of China (No. 31860714), and National Natural Science Foundation of China (No. 31871718), and the APC was funded by National Key Research and Development Program of China (No. 2021YFD1600702-3).