Muscle fiber characteristics and expression level of Troponin T3, Toll-like receptor 2, and Toll-like receptor 4 genes in chicken meat with white striping

Antika Boonlaos; Muhammad Jasim Uddin; Katchaporn Temyord; Danai Jattawa; Autchara Kayan

doi:10.14202/vetworld.2023.1415-1420

Muscle fiber characteristics and expression level of Troponin T3, Toll-like receptor 2, and Toll-like receptor 4 genes in chicken meat with white striping

Vet World. 2023;16(7):1415-1420. doi: 10.14202/vetworld.2023.1415-1420. Epub 2023 Jul 9.

Authors

Antika Boonlaos¹, Muhammad Jasim Uddin^{2

3}, Katchaporn Temyord⁴, Danai Jattawa¹, Autchara Kayan¹

Affiliations

¹ Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand.
² School of Veterinary Medicine, Murdoch University, Western Australia, Australia.
³ Center for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Murdoch, Australia.
⁴ Bureau of Livestock Standard and Certification, Department of Livestock Development, Bangkok, Thailand.

Abstract

Background and aim: The poultry industry faces an emerging muscular defect in chicken meat called white striping (WS). The biological processes associated with WS myopathy are immune system activation, angiogenesis, hypoxia, cell death, and striated muscle contraction. We examined the Troponin T3 (TNNT3), Toll-like receptor 2 (TLR2), and Toll-like receptor 4 (TLR4) genes based on their functions related to muscle contraction and the innate immune system. This study aimed to determine the muscle fiber characteristics (MFCs) and expression level of TNNT3, TLR2, and TLR4 genes in white striping chicken meat (WSCM).

Materials and methods: A total of 428 breast samples were randomly collected from a commercial poultry processing plant. The samples were classified into four levels: 0 (normal), 1 (moderate WS), 2 (severe WS), and 3 (extreme WS). Five samples per group were selected to evaluate MFCs, including total number of muscle fibers, muscle fiber diameter, cross-sectional area, endomysium thickness, and perimysium thickness. Five samples per group were selected for ribonucleic acid (RNA) isolation to evaluate the messenger RNA (mRNA) expression levels of TNNT3, TLR2, and TLR4 genes related to WS.

Results: Statistical analysis revealed that the total number of fibers, endomysium thickness, and perimysium thickness significantly differed between groups (p < 0.05). Muscle fiber diameter and cross-sectional area did not significantly differ (p > 0.05). The expression of the TNNT3 gene did not significantly differ among groups (p > 0.05). Toll-like receptor 2 and TLR4 mRNA expression significantly differed among groups (p < 0.05).

Conclusion: These detailed MFCs will provide baseline information to observe WS in chicken meat. Toll-like receptor 2 and TLR4 genes may play a role in the occurrence of WS in chicken meat through non-specific immune reactions.

Keywords: chicken; gene expression; myopathy; poultry; white striping.