Biological functions of endophytic bacteria in Robinia pseudoacacia 'Hongsen '

Minqing Huang; Lijing Chen; Jiasi Ma; Jingzhi Mo; Lu He; Qihua Liang; Guixiang Peng; Zhiyuan Tan

doi:10.3389/fmicb.2023.1128727

Biological functions of endophytic bacteria in Robinia pseudoacacia 'Hongsen '

Front Microbiol. 2023 Aug 9:14:1128727. doi: 10.3389/fmicb.2023.1128727. eCollection 2023.

Authors

Minqing Huang¹, Lijing Chen¹, Jiasi Ma¹, Jingzhi Mo¹, Lu He¹, Qihua Liang², Guixiang Peng², Zhiyuan Tan¹

Affiliations

¹ College of Agriculture, South China Agricultural University, Guangzhou, China.
² College of Natural Resources and Environment, South China Agricultural University, Guangzhou, China.

Abstract

Introduction: Endophytes and their host plants have co-evolved for a very long time. This relationship has led to the general recognition of endophytes as a particular class of microbial resources. R. pseudoacacia 'Hongsen' is drought- and barren-resistant species that can be grown in both the north and south of China, efficiently addresses the ecological issues caused by China's 'southern eucalyptus and northern poplar. Up to date, cultured-dependent studies are available for the R. pseudoacacia nitrogen-fixing and other endophytes. Therefore, the present research studied the R. pseudoacacia 'Hongsen,' microbiome in detail by high-throughput sequencing and culture dependant.

Methods: This study examined microbial species and functional diversity in Robinia pseudoacacia 'Hongsen' using culture-dependent (isolation) and culture-independent techniques.

Results: A total of 210 isolates were isolated from R. pseudoacacia 'Hongsen.' These isolates were clustered into 16 groups by the In Situ PCR (IS-PCR) fingerprinting patterns. 16S rRNA gene sequence analysis of the representative strain of each group revealed that these groups belonged to 16 species of 8 genera, demonstrating the diversity of endophytes in R. pseudoacacia 'Hongsen'. 'Bacillus is the most prevalent genus among all the endophytic bacteria. High-throughput sequencing of endophytic bacteria from R. pseudoacacia 'Hongsen' of the plant and the rhizosphere soil bacteria showed that the bacterial populations of soil near the root, leaf, and rhizosphere differed significantly. The microbial abundance decreased in the endophytes as compared to the rhizosphere. We observed a similar community structure of roots and leaves. With and without root nodules, Mesorhizobium sp. was significantly different in R. pseudoacacia 'Hongsen' plant.

Discussion: It was predicted that R. pseudoacacia 'Hongsen' plant endophytic bacteria would play a significant role in the metabolic process, such as carbohydrate metabolism, amino acid metabolism, membrane transport, and energy metabolism.

Keywords: Robinia pseudoacacia ‘Hongsen’; biodiversity and biological function; endophytic bacteria; high-throughput sequencing; rhizosphere soil bacteria.