Spatial nanopores promote laccase degradation of bisphenol A and its analogs

Mei Du; Jingzhang Liu; Bang Huang; Qiong Wang; Fengbang Wang; Lei Bi; Chunyan Ma; Maoyong Song; Guibin Jiang

doi:10.1016/j.scitotenv.2023.166429

Spatial nanopores promote laccase degradation of bisphenol A and its analogs

Sci Total Environ. 2023 Nov 25:901:166429. doi: 10.1016/j.scitotenv.2023.166429. Epub 2023 Aug 22.

Authors

Mei Du¹, Jingzhang Liu¹, Bang Huang², Qiong Wang¹, Fengbang Wang¹, Lei Bi¹, Chunyan Ma¹, Maoyong Song³, Guibin Jiang¹

Affiliations

¹ State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
² State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing 100085, China; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances, Institute of Environment and Health, Jianghan University, 430056 Wuhan, China.
³ State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: mysong@rcees.ac.cn.

PMID: 37619739
DOI: 10.1016/j.scitotenv.2023.166429

Abstract

Bisphenol A (BPA) and its analogs are endocrine-disrupting chemicals that are frequently detected in environmental and human samples. However, the effective removal of BPA and its analogs has not yet been extensively studied. Herein, we introduce a novel enzyme reactor for the degradation of BPA and its analogs in water. The influence of pore size on the degradation efficiency of immobilized laccase in the spatial nanopores of hydrogel was investigated using BPA as a representative compound. This showed that nanopores enhance the activity of immobilized laccases in a pore size-dependent manner and increase their stability. Compared with the same amount of free laccase, the 50 mg/L BPA degradation performance of laccase immobilized in 76 nm nanopores increased to 300 %. Taking advantage of magnetic separation, this immobilized laccase can be reused, and its degradation capacity was maintained at over 73.7 % after ten reactions. Moreover, the degradation of seven BPA analogs was 1.03-5.88 times higher using laccase immobilized in nanopores compared with free laccase. Also, the biocatalyst could efficiently degrade BPA analogs in real water matrix. This study opens up a new avenue for the removal of BPA and its analogs by immobilizing laccase in nanopores, overcoming the key limitations introduced by the short enzyme life span and non-reusability.

Keywords: Bisphenol A; Bisphenol A analogs; Degradation; Laccase; Nanopore.