Objective: To investigate the effects of acupuncture on JNK pathway and autophagy level in rats with intracerebral hemorrhage （ICH） and explore the partial mechanism of acupuncture against ICH.
Methods: SD rats were randomly divided into blank group, model group and acupuncture group. Each group was divided into Day 1, Day 3 and Day 7 subgroups respectively, with 5 rats in each group. The autologous blood injection was adopted to duplicate rat model of ICH. In the acupuncture group, the needle was inserted from "Baihui" （GV20） towards "Qubin" （GB7） on the affected side, stimulating for 30 min each time, once daily； the same acupuncture technique was opera-ted in each subgroup for 1, 3 and 7 days, separately. Using Bederson scale, the neurological deficit was evaluated in each group. Western blot was adopted to detect the protein expression levels of Beclin1, LC3Ⅰ/Ⅱ, phosphorylated c-Jun amino-terminal kinase （p-JNK） and the phosphorylated （p）-c-Jun around hematoma lesion of the brain tissue of rats in each group.
Results: After treatment, the neurological deficit score of rats in the model group was higher than that of the blank group at each time point （P<0.05）, and the score of the acupuncture group started declining since the 3rd day of treatment when compared with the model group （P<0.05）. At each time point, compared with the blank group, the protein expression levels of LC3Ⅰ/Ⅱ, Beclin1, p-c-Jun and p-JNK was increased （P<0.01）. Compared with the model group, the protein expression level of LC3Ⅰ/Ⅱ was reduced （P<0.05）； the protein expression levels of Beclin1, p-c-Jun and p-JNK was increased （P<0.05, P<0.01） on day 3 and 7 in the acupuncture group.
Conclusion: Acupuncture can activate the JNK pathway in the brain tissue of rats with ICH and increase the level of autophagy, thereby improving the neurological function of the rats with ICH.
目的：观察针刺对脑出血大鼠JNK通路及自噬水平的影响，探讨针刺抗脑出血的部分机制。方法：SD大鼠随机分为空白组、模型组、针刺组，每组进一步分为1、3、7 d亚组，每个亚组5只。采用自体血注入法复制脑出血大鼠模型。针刺组针刺“百会”透患侧“曲鬓”，每次30 min，每日1次，各亚组分别治疗1、3、7 d。采用Bederson评分评价各组大鼠神经功能缺损情况；Western blot法检测各组大鼠出血脑组织B淋巴细胞瘤-2基因同源结构域蛋白抗体（Beclin1）、微管相关蛋白轻链3（LC3）Ⅰ/Ⅱ、磷酸化c-Jun氨基末端激酶（p-JNK）、磷酸化（p）-c-Jun蛋白相对表达量。结果：各时点模型组大鼠Bederson评分高于空白组（P<0.05），针刺组大鼠Bederson评分3、7 d低于模型组（P<0.05）。与空白组比较，模型组各时间点的LC3Ⅰ/Ⅱ、Beclin1、p-c-Jun、p-JNK蛋白表达升高（P<0.01）。与模型组比较，针刺组大鼠3、7 d时LC3Ⅰ/Ⅱ相对表达量降低（P<0.05，P<0.01），Beclin1、p-c-Jun、p-JNK蛋白表达均升高（P<0.05，P<0.01）。结论：针刺可激活脑出血大鼠脑组织中JNK通路提高自噬水平，从而改善脑出血大鼠神经功能。.
Keywords: Acupuncture; Autophagy; Intracerebral hemorrhage; c-Jun amino-terminal kinase-pathway.