Enhancing food waste reduction efficiency and high-value biomass production in Hermetia illucens rearing through bioaugmentation with gut bacterial agent

Yizhan Qiu; Pan Wang; Yuwen Guo; Luxi Zhang; Jiaxin Lu; Lianhai Ren

doi:10.1016/j.scitotenv.2023.166488

Enhancing food waste reduction efficiency and high-value biomass production in Hermetia illucens rearing through bioaugmentation with gut bacterial agent

Sci Total Environ. 2023 Dec 15:904:166488. doi: 10.1016/j.scitotenv.2023.166488. Epub 2023 Aug 21.

Authors

Yizhan Qiu¹, Pan Wang², Yuwen Guo³, Luxi Zhang¹, Jiaxin Lu⁴, Lianhai Ren⁵

Affiliations

¹ School of Ecology and Environment, Beijing Technology and Business University, Beijing 100048, China.
² School of Ecology and Environment, Beijing Technology and Business University, Beijing 100048, China; State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing 100048, China; Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing 100048, China.
³ AnronX Technology (Beijing) Joint Stock Co., Ltd., Beijing 100086, China.
⁴ School of Ecology and Environment, Beijing Technology and Business University, Beijing 100048, China; State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing 100048, China; Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing 100048, China. Electronic address: lujiaxin@btbu.edu.cn.
⁵ School of Ecology and Environment, Beijing Technology and Business University, Beijing 100048, China; State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing 100048, China; Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing 100048, China. Electronic address: renlh@th.btbu.edu.cn.

PMID: 37611705
DOI: 10.1016/j.scitotenv.2023.166488

Abstract

The black soldier fly (BSF) rearing technology has been a promising bioconversion method for food waste (FW) disposal. However, when used independently, it currently only achieves low efficiency and biomass transformation rates (BTR). This study screened and identified two strains of gut beneficial bacteria, Bacillus cereus and Bacterium YC-LK-LKJ45. The efficiency of a complex culture formulated by these strains was investigated, focusing on enhancing FW reduction and high-value biomass production during the rearing of BSF larvae. The coculture agent group (G1-10%, with two strains in 1:1 volume ratio at a 10 % dosage) exhibited higher larval yield (627.67 g·kg^-1), BTR (47.90 %), FW reduction efficiency (80.67 %), and total protein and fat yield (261.99 g·kg^-1and 46.24 g·kg^-1) compared to the control and the monoculture agent group (which added a single gut beneficial bacteria agent, either Bacillus cereus or Bacterium YC-LK-LKJ45). The bacterial agent altered the richness and diversity of the gut microbial community of BSF, increasing the relative abundance of beneficial bacteria such as Bacillus, Oceano bacillus, and Akkermansia, while decreasing pathogenic bacteria, such as Acinetobacter and Escherichia-Shigella. Structural equation model quantification revealed that α-diversity (λ = 0.897, p < 0.001) and BTR (λ = 0.747, p < 0.001) are crucial drivers for enhancing high-value biomass during bioaugmentation rearing. This investigation provides a theoretical framework for the effective management of food waste using BSF, enhancing its decomposition and transformation into higher-value biomass.

Keywords: Bioaugmentation; Bioconversion; Food waste; Gut microbial community; Hermetia illucens.

MeSH terms

Animals
Bacillus*
Bacteria
Biomass
Diptera* / microbiology
Food
Gastrointestinal Microbiome*
Larva / metabolism
Refuse Disposal*