Profiling Host MicroRNA Responses to Henipavirus Infection

Ryan J Farr; Sudeep Dahal; Leon Tribolet; Andrew G D Bean; Christopher Cowled; Cameron R Stewart

doi:10.1007/978-1-0716-3283-3_19

Profiling Host MicroRNA Responses to Henipavirus Infection

Methods Mol Biol. 2023:2682:261-279. doi: 10.1007/978-1-0716-3283-3_19.

Authors

Ryan J Farr¹, Sudeep Dahal¹, Leon Tribolet¹, Andrew G D Bean¹, Christopher Cowled¹, Cameron R Stewart²

Affiliations

¹ CSIRO Australian Animal Health Laboratory, Health & Biosecurity, Geelong, VIC, Australia.
² CSIRO Australian Animal Health Laboratory, Health & Biosecurity, Geelong, VIC, Australia. Cameron.Stewart@csiro.au.

PMID: 37610588
DOI: 10.1007/978-1-0716-3283-3_19

Abstract

Diseases caused by henipaviruses feature incubation periods of up to 16 days, during which infected animals may show no apparent signs of disease yet be capable of transmitting the virus to humans. This risk has prompted research into host-derived biomarkers for early disease detection. Here, we describe a methodology for the assaying of host microRNAs (miRs), small non-coding RNAs that show promise as biomarkers for several human diseases and are responsive during early-stage henipavirus infection. In addition to their potential as disease biomarkers, miRNA profiling of henipavirus infections provides insight into cellular and immune pathways associated with disease pathogenesis.

Keywords: Biomarker; Diagnostic; Henipavirus; microRNA.

MeSH terms

Animals
Biological Assay
Henipavirus Infections*
Humans
MicroRNAs* / genetics

Substances

MicroRNAs