Prediction of gastroesophageal reflux episodes by smooth muscle electromyography: A translational study in rats and adolescents

Anikó Nagy; Kálmán F Szűcs; György Grosz; Miklós Süle; Ferenc Fekete; Anna Karoliny; Mariann Borsos; Zsuzsanna Papp; Dóra Vigh; Róbert Gáspár

doi:10.1016/j.heliyon.2023.e18859

Prediction of gastroesophageal reflux episodes by smooth muscle electromyography: A translational study in rats and adolescents

Heliyon. 2023 Aug 9;9(8):e18859. doi: 10.1016/j.heliyon.2023.e18859. eCollection 2023 Aug.

Authors

Affiliations

¹ Heim Pal National Pediatric Institute, Budapest, Hungary.
² Department of Pharmacology and Pharmacotherapy, Albert-Szent-Györgyi Medical School, Faculty of Medicine, University of Szeged, Szeged, Hungary.
³ MSB-MET Ltd, Balatonfüred, Hungary.
⁴ Adware Research Ltd, Balatonfüred, Hungary.

Abstract

Aims: Our aim was to measure the myoelectric modifications during gastric acid secretion along with the gastric pH in a rat model and to detect the gastrointestinal (GI) myoelectric changes in adolescents suffering from gastroesophageal reflux disease (GERD) along with the esophageal pH measurement.

Main methods: In anesthetized rats, gastric acid secretion was initiated with intragastric histamine (50 mg/kg), and gastric pH, GI myoelectric activity and mechanical GI contractions were measured with intragastric pH electrode, subcutaneously implanted smooth muscle electromyography (SMEMG) electrodes and organ implanted strain gauges, respectively. In the clinical study, esophageal pH and GI myoelectric activity were measured in adolescents suffering from GERD with intraesophageal pH electrode and SMEMG electrodes placed on the abdominal surface, respectively. The SMEMG records were analyzed by fast Fourier transformation (FFT) and power spectrum density maximum (PsD_max) values were calculated for the GI segments.

Key findings: In rats, histamine initiated an immediate increase in gastric PsD_max, which preceded the significant reduction in gastric pH by 75 min. The myoelectric change was independent of mechanical GI contractions. In adolescents, the GERD episodes were preceded by a significant increase in gastric PsD_max 45 min earlier. These changes were independent of motion or meals.

Significance: Increased gastric myoelectric activity during histamine stimulation or GERD might be linked to the enhanced activity of the gastric proton pump, indicating a link between gastric acid secretion and GERD episodes. It is supposed that SMEMG might be a tool for predicting forthcoming reflux episodes in GERD.

Keywords: Adolescents; Electromyography; GERD; Gastrointestinal; Rat; pH.