Development of an immunochromatographic assay for the rapid screening of torasemide in health food

Jieyu Yan; Aihong Wu; Liqiang Liu; Liguang Xu; Hua Kuang; Chuanlai Xu; Lingling Guo

doi:10.1016/j.foodchem.2023.137166

Development of an immunochromatographic assay for the rapid screening of torasemide in health food

Food Chem. 2024 Jan 30:432:137166. doi: 10.1016/j.foodchem.2023.137166. Epub 2023 Aug 18.

Authors

Jieyu Yan¹, Aihong Wu¹, Liqiang Liu¹, Liguang Xu¹, Hua Kuang¹, Chuanlai Xu², Lingling Guo³

Affiliations

¹ State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
² State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: xcl@jiangnan.edu.cn.
³ State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: gling0329@jiangnan.edu.cn.

PMID: 37607444
DOI: 10.1016/j.foodchem.2023.137166

Abstract

Torasemide is a new loop diuretic agent added illegally to health foods for weight loss, which can result in serious health risks for consumers. A rapid and sensitive immunochromatographic assay for detection of torasemide (ICA) based on a new monoclonal antibody (mAb) was developed. The mAb IC₅₀ for torasemide was 0.93 ng/mL, and the mAb did not cross-react with other analogues. In PBS, the cut-off value and limit of detection were 1 ng/mL and 0.11 ng/mL, respectively, with a linear range between 0.61 and 6.13 ng/mL. In slimming tablet and capsule samples, the cut-off value was 5 ng/g. Recoveries were 101.1% ± 1.7%-106.1% ± 1.3% in tablet samples and 101.2% ± 2.2%-109.1% ± 3.9% in capsule samples, with coefficients of variation 2.1%-3.1% and 1.8%-3.6%, respectively, consistent with existing LC-MS/MS methods. Therefore, the ICA is suitable for use in slimming tablet and capsule samples.

Keywords: Health food; Immunochromatographic assay; Monoclonal antibody; Torasemide.

MeSH terms

Antibodies, Monoclonal
Chromatography, Liquid
Foods, Specialized*
Immunoassay
Tandem Mass Spectrometry*
Torsemide

Substances

Torsemide
Antibodies, Monoclonal