Discovery of Tetronate-Containing Kongjuemycins from a Coral-Associated Actinomycete and Elucidation of Their Biosynthetic Origin

Zhuangjie Fang; Qingbo Zhang; Weiliang Xiong; Lili Sun; Bin Tan; Mengyi Zhu; Liang Ma; Liping Zhang; Yiguang Zhu; Changsheng Zhang

doi:10.1021/acs.orglett.3c02231

Discovery of Tetronate-Containing Kongjuemycins from a Coral-Associated Actinomycete and Elucidation of Their Biosynthetic Origin

Org Lett. 2023 Sep 1;25(34):6346-6351. doi: 10.1021/acs.orglett.3c02231. Epub 2023 Aug 22.

Authors

Zhuangjie Fang^{1

2}, Qingbo Zhang^{1

2

3}, Weiliang Xiong¹, Lili Sun^{1

2}, Bin Tan¹, Mengyi Zhu¹, Liang Ma^{1

3}, Liping Zhang^{1

3}, Yiguang Zhu^{1

2

3}, Changsheng Zhang^{1

2

3}

Affiliations

¹ Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
² University of Chinese Academy of Sciences, Beijing 100049, China.
³ Sanya Institute of Ocean Eco-Environmental Engineering, Yazhou Scientific Bay, Sanya 572000, China.

PMID: 37606755
DOI: 10.1021/acs.orglett.3c02231

Abstract

Tetronate antibiotics make up a growing family of natural products with a wide variety of biological activities. Herein, we report four new tetronates kongjuemycins (KJMs, 5-8) from a coral-associated actinomycete Pseudonocardia kongjuensis SCSIO 11457, and the identification and characterization of the KJM biosynthetic gene cluster (kjm) by heterologous expression, comparative genomic analysis, isotope labeling, and gene knockout studies. The biosynthesis of KJMs is demonstrated to harness diverse precursors from primary metabolism for building secondary metabolites.