Methods For Visualizing Intracellular Organelles

J Vis Exp. 2023 Mar 3:(193):10.3791/64966. doi: 10.3791/64966.

Abstract

Aishwarya, R. et al. Visualizing subcellular localization of a protein in the heart using quantum dots-mediated immuno-labeling followed by transmission electron microscopy. Journal of Visualized Experiments. (187), e64085 (2022). Liu, B. et al. Visualizing mitophagy with fluorescent dyes for mitochondria and lysosome. Journal of Visualized Experiments. (189), e64647 (2022). Liu, C. Y., Zhu, J., Xie, Z. Visualizing yeast organelles with fluorescent protein markers. Journal of Visualized Experiments. (182), e63846 (2022). Zhao, X., Guo, S. Antibody uptake assay for tracking Notch/Delta endocytosis during the asymmetric division of zebrafish radial glia progenitors. Journal of Visualized Experiments. 65030 (2022). Park, S., Zhuang, Y., Shi, X. Label-retention expansion microscopy (LR-ExM) enables super-resolution imaging and high-efficiency labeling. Journal of Visualized Experiments. (188), e63793 (2022).

Publication types

  • Video-Audio Media
  • Editorial
  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural
  • Comment

MeSH terms

  • Animals
  • Fluorescent Dyes
  • Lysosomes*
  • Microscopy, Electron, Transmission
  • Mitochondria
  • Zebrafish*

Substances

  • Fluorescent Dyes