Methods of Isolation and Purification of Extracellular Vesicles from Different Biological Matrixes: Special Issue at a Glance

Gemma Chiva-Blanch

doi:10.3791/64963

Methods of Isolation and Purification of Extracellular Vesicles from Different Biological Matrixes: Special Issue at a Glance

J Vis Exp. 2023 Feb 10:(192). doi: 10.3791/64963.

Author

Gemma Chiva-Blanch¹

Affiliation

¹ Department of Endocrinology and Nutrition August Pi i Sunyer Biomedical Research Institute - IDIBAPS, Hospital Clínic of Barcelona; Biomedical Network Research Centre on Obesity and Nutrition Physiopathology (CIBEROBN), Instituto de Salud Carlos III; gchiva@recerca.clinic.cat.

PMID: 37602863
DOI: 10.3791/64963

Abstract

Hanine El Itawi, H., et al. Isolation of Splenic Microvesicles in a Murine Model of Intraperitoneal Bacterial Infection. J Vis Exp. 186, DOI: 10.3791/63480 (2022) Jones, M.T., Manioci S.W., S1, Russell, A.E. Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media. J Vis Exp. 186, DOI: 10.3791/63614 (2022) Ryan, J.M., Dobos, K.M., Kruh-Garcia, N.A. Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography. J Vis Exp. 186, DOI: 10.3791/63895 (2022) Valle-Tamayo, N., et al. Enrichment of Astrocyte-Derived Extracellular Vesicles from Human Plasma J Vis Exp. 186, DOI: 10.3791/64107 (2022) Koh, B., et al. A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells J Vis Exp. 186, DOI: 10.3791/64106 (2022).

Publication types

Editorial
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Astrocytes
Cell-Derived Microparticles*
Chromatography, Gel
Culture Media, Conditioned
Extracellular Vesicles*
Humans
Mice

Substances

Culture Media, Conditioned