Banana disease-suppressive soil drives Bacillus assembled to defense Fusarium wilt of banana

Huacai Fan; Ping He; Shengtao Xu; Shu Li; Yongfen Wang; Wenlong Zhang; Xundong Li; Hui Shang; Li Zeng; Si-Jun Zheng

doi:10.3389/fmicb.2023.1211301

Banana disease-suppressive soil drives Bacillus assembled to defense Fusarium wilt of banana

Front Microbiol. 2023 Aug 3:14:1211301. doi: 10.3389/fmicb.2023.1211301. eCollection 2023.

Authors

Huacai Fan¹, Ping He^{1

2}, Shengtao Xu¹, Shu Li¹, Yongfen Wang^{1

3}, Wenlong Zhang^{1

2}, Xundong Li¹, Hui Shang¹, Li Zeng¹, Si-Jun Zheng^{1

4}

Affiliations

¹ Yunnan Key Laboratory of Green and Control of Agricultural Transboundary Pests, Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, China.
² State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, College of Plant Protection, Yunnan Agricultural University, Kunming, China.
³ Institute of Tropical and Subtropical Industry Crops, Yunnan Academy of Agricultural Sciences, Baoshan, China.
⁴ Bioversity International, Kunming, China.

Abstract

Fusarium wilt of banana (FWB) caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), poses a serious problem for sustainable banana production. Biological control is one of the effective measures to control this destructive disease. High-throughput sequencing of soil microorganisms could significantly improve the efficiency and accuracy of biocontrol strain screening. In this study, the soil microbial diversity of six main banana-producing areas in Yunnan was sequenced by Illumina Miseq platform. The outcome of this study showed the genus of Chujaibacter, Bacillus, and Sphingomonas were significantly enriched in microorganism community composition. Further correlation analysis with soil pathogen (Foc TR4) content showed that Bacillus was significantly negatively correlated with pathogen content. Therefore, we isolated and identified Bacillus from the disease-suppressive soils, and obtained a B. velezensis strain YN1910. In vitro and pot experiments showed that YN1910 had a significant control effect (78.43-81.76%) on banana Fusarium wilt and had a significant growth promotion effect on banana plants.

Keywords: Bacillus spp.; Foc TR4; Fusarium wilt of banana; biological control; soil microorganism diversity.