Variable promotion of algae and macrophyte organic matter on methanogenesis in anaerobic lake sediment

Tong Wang; Maidina Zhumabieke; Nan Zhang; Cheng Liu; Jicheng Zhong; Qianjiahua Liao; Lei Zhang

doi:10.1016/j.envres.2023.116922

Variable promotion of algae and macrophyte organic matter on methanogenesis in anaerobic lake sediment

Environ Res. 2023 Nov 15;237(Pt 1):116922. doi: 10.1016/j.envres.2023.116922. Epub 2023 Aug 19.

Authors

Tong Wang¹, Maidina Zhumabieke², Nan Zhang³, Cheng Liu⁴, Jicheng Zhong⁴, Qianjiahua Liao⁵, Lei Zhang⁶

Affiliations

¹ State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, 210008, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
² Department of Environmental Science, China Pharmaceutical University, Nanjing, 211198, PR China.
³ State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, 210008, PR China; School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing, 210044, PR China.
⁴ State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, 210008, PR China.
⁵ Department of Environmental Science, China Pharmaceutical University, Nanjing, 211198, PR China. Electronic address: lqjh@cpu.edu.cn.
⁶ State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, 210008, PR China. Electronic address: leizhang@niglas.ac.cn.

PMID: 37598844
DOI: 10.1016/j.envres.2023.116922

Abstract

Shallow lakes are an important natural source of atmospheric methane (CH₄), and the input of autochthonous organic matter (OM) into their sediments encourages methanogenesis. Although algal- and macrophytic-originated OM in these lakes are expected to have different impacts on methanogenesis and methanogenic archaeal communities in lake sediments owing to their various properties, their specific influence and role in sediment remain unclear. In this study, a 148-day incubation was carried out by adding algal- and macrophytic-OM to the sediments of shallow eutrophic Lake Chaohu and Lake Taihu in China. CH₄ was periodically monitored, while the methanogens were examined via qPCR and high-throughput sequencing at the end of incubation. Algal-OM stimulated CH₄ production more than macrophytic-OM in both sediments, with the rates initially increasing and then decreasing before reaching a relative constant. Macrophytic-OM promoted CH₄ production to a comparable extent in both lakes, while algal-OM promoted greater CH₄ in Lake Chaohu than in Lake Taihu. However, algal-OM did not significantly increase mcrA gene copies, while macrophytic-OM did by 17.0-20.1-fold. Algal-OM potentially promoted the methylotrophic pathway in Lake Taihu but did not change the methanogenic structure in Lake Chaohu. Comparatively, macrophytic-OM promoted CH₄ production mainly by acetoclastic methanogen proliferation in both lakes. More CH₄ release with algal-OM compared to macrophytic-OM deserves further attention owing to the prevailing increasing algal blooms and the declining macrophyte population in lakes.

Keywords: Eutrophication; Lake Chaohu; Lake Taihu; Methane (CH(4)); Methanogens.