High-throughput differential scanning fluorimetry (DSF) and cellular thermal shift assays (CETSA): Shifting from manual to automated screening

Catherine S Hansel; Alice Lanne; Hannah Rowlands; Joseph Shaw; Matthew J Collier; Helen Plant

doi:10.1016/j.slast.2023.08.004

High-throughput differential scanning fluorimetry (DSF) and cellular thermal shift assays (CETSA): Shifting from manual to automated screening

SLAS Technol. 2023 Dec;28(6):411-415. doi: 10.1016/j.slast.2023.08.004. Epub 2023 Aug 19.

Authors

Catherine S Hansel¹, Alice Lanne², Hannah Rowlands², Joseph Shaw³, Matthew J Collier², Helen Plant²

Affiliations

¹ High-throughput Screening, Hit Discovery, Discovery Sciences, R&D, AstraZeneca, Alderley Park, UK. Electronic address: katie.hansel@astrazeneca.com.
² High-throughput Screening, Hit Discovery, Discovery Sciences, R&D, AstraZeneca, Alderley Park, UK.
³ Assay Development, Discovery Biology, Discovery Sciences, R&D, AstraZeneca, Cambridge, UK.

PMID: 37598756
DOI: 10.1016/j.slast.2023.08.004

Abstract

Biophysical affinity screening is increasingly being adopted as a high-throughput hit finding technique in drug discovery. Automation is highly beneficial to high-throughput screening (HTS) since a large number of compounds need to be reproducibly tested against a biological target. Herein, we describe how we have automated two biophysical affinity screening methods that rely on a thermal shift in protein melting temperature upon small molecule binding: differential scanning fluorimetry (DSF) and the cellular thermal shift assay (CETSA).

Keywords: Automation; High-throughput screening; Thermal shift assays.

MeSH terms

Drug Discovery* / methods
Fluorometry / methods
High-Throughput Screening Assays* / methods
Proteins / metabolism
Temperature

Substances

Proteins