The green production of silver nanoparticles (AgNPs) produces AgNPs with minimum influence on the environment by using plant components such as alkaloids, carbohydrates, lipids, enzymes, flavonoids, terpenoids, and polyphenols as reducing agents. In the present investigation, Azadirachta indica leaf extract was used to form AgNPs from a 1 mM silver nitrate solution. The plan proved to be incredibly straightforward, cost-effective, and effective. The production of the nanoparticles was observed visually, where the colorless fluid turns into a brown-colored solution. Further research was carried out using x-ray diffraction, Fourier-transform infrared analysis, scanning electron microscopy, and transmission electron microscopy (TEM) in addition to UV-visible spectroscopy. The size range of AgNPs determined by TEM was 10-30 nm. When the diffusion technique was employed to demonstrate the antibacterial effect of AgNPs on various pathogens, the zones of inhibition for Staphylococcus aureus, Bacillus cereus, and Escherichia coli, when 50 g of AgNPs were used were 16, 12, and 17 mm, respectively. By examining the leakage of reducing sugars and proteins, the mechanism by which nanoparticle antibacterial properties were explored, showed that AgNPs were capable of lowering membrane permeability.
Keywords: Azadirachta indica; antibacterial; characterization; green synthesis; molecular docking; silver nanoparticles.
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