Synthesis of a Au/Au NPs-PPy/l-CYs/ZIF-8 nanocomposite electrode for voltammetric determination of insulin in human blood

Jamal Kouhdareh; Rahman Karimi-Nami; Hassan Keypour; Khadijeh Rabiei; Sedigheh Alavinia; Shokoufeh Ghahri Saremi; Mohammad Noroozi

doi:10.1039/d3ra04064j

Synthesis of a Au/Au NPs-PPy/l-CYs/ZIF-8 nanocomposite electrode for voltammetric determination of insulin in human blood

RSC Adv. 2023 Aug 16;13(35):24474-24486. doi: 10.1039/d3ra04064j. eCollection 2023 Aug 11.

Authors

Jamal Kouhdareh¹, Rahman Karimi-Nami², Hassan Keypour¹, Khadijeh Rabiei³, Sedigheh Alavinia¹, Shokoufeh Ghahri Saremi⁴, Mohammad Noroozi⁵

Affiliations

¹ Faculty of Chemistry, Bu-Ali Sina University Hamedan 65174 Iran haskey1@yahoo.com.
² Department of Chemistry, Faculty of Science, University of Maragheh Maragheh Iran.
³ Department of Chemistry, Faculty of Science, Qom University of Technology Qom Iran.
⁴ Department of Chemistry, Payame Noor University Tehran Iran.
⁵ Center for Research and Development of Petroleum Technologies at Kermanshah, Research Institute of Petroleum Industry (RIPI) Iran.

Abstract

In this work, a modified electrode named Au/Au NPs-PPy/l-CYs/ZIF-8 was designed and built and simultaneously doped into electropolymerized polypyrrole (PPy) film using cyclic voltammetry (CV). Scanning Electron Microscopy (SEM), Electrochemical Impedance Spectroscopy (EIS), and CV were used to characterize the composite films. The PPy-(ZIF-8) modified Au electrode was used to determine insulin using Square-Wave Voltammetry (SWV). It was found that the prepared zeolitic imidazolate framework-8 had excellent electrocatalytic activity towards insulin oxidation due to its unique properties. The oxidation peak current of insulin hormone increased with its concentration in the range from 1.0 to 60 nM with the linear regression equation: I_pa = 0.3421C (nM) + 3.2762 (γ = 0.998). The measurement limit was estimated to be 1 nM. While the common coexisting substances showed no interference in the response of the modified electrode to insulin, the modified electrode indicated reproducible behavior and a high level of stability during the experiments. The advantages of using these nanocomposites on the surface of modified electrodes include increased stability, good interaction between the analyte and the modified electrode, conductivity, and excellent performance due to the nanometer size of the composites. As a result, it may be particularly suitable for analytical purposes.