Separation of cyclotron-produced cobalt-55/58m from iron targets using cation exchange chromatography with non-aqueous solvents and extraction chromatography

Wilson Lin; Eduardo Aluicio-Sarduy; Kendall E Barrett; Todd E Barnhart; Jason C Mixdorf; Molly C DeLuca; Jonathan W Engle

doi:10.1016/j.apradiso.2023.110980

Separation of cyclotron-produced cobalt-55/58m from iron targets using cation exchange chromatography with non-aqueous solvents and extraction chromatography

Appl Radiat Isot. 2023 Oct:200:110980. doi: 10.1016/j.apradiso.2023.110980. Epub 2023 Aug 12.

Authors

Wilson Lin¹, Eduardo Aluicio-Sarduy², Kendall E Barrett², Todd E Barnhart², Jason C Mixdorf², Molly C DeLuca², Jonathan W Engle³

Affiliations

¹ Department of Medical Physics, University of Wisconsin, 1111 Highland Ave., Madison, WI, 53705, United States. Electronic address: wlin99@wisc.edu.
² Department of Medical Physics, University of Wisconsin, 1111 Highland Ave., Madison, WI, 53705, United States.
³ Department of Medical Physics, University of Wisconsin, 1111 Highland Ave., Madison, WI, 53705, United States; Department of Radiology, University of Wisconsin, 600 Highland Ave., Madison, WI, 53792, United States.

PMID: 37591186
PMCID: PMC10529958 (available on 2024-10-01)
DOI: 10.1016/j.apradiso.2023.110980

Abstract

Cobalt-55 and -58m form a theranostic pair that has relevant properties for cancer research. We report a cation exchange chromatography/extraction chromatography method that separates cyclotron-produced ^55/58mCo from ^54/57Fe in <1.5 h, recovers >85% Co and achieves [⁵⁵Co]Co-NOTA and -DOTA AMA 89 ± 48 and 35 ± 7 MBq/nmol (EOB), respectively. Cobalt-55 and -58m were quantitatively labeled to functionalized NOTA at 106 and 50 MBq/nmol (EOB), respectively, corroborating measured AMA. This method is faster than previously published methods and achieves better [^55/58mCo]Co-NOTA and -DOTA AMA.

Keywords: Cation exchange chromatography; Cobalt-55/58m; Nuclear medicine.

Grants and funding

P01 CA250972/CA/NCI NIH HHS/United States