Immunomodulatory effects of one novel microRNA miR-63 in pearl oyster Pinctada fucata martensii

Meiqi Dai; Yuting Zhang; Yu Jiao; Yuewen Deng; Xiaodong Du; Chuangye Yang

doi:10.1016/j.fsi.2023.109002

Immunomodulatory effects of one novel microRNA miR-63 in pearl oyster Pinctada fucata martensii

Fish Shellfish Immunol. 2023 Sep:140:109002. doi: 10.1016/j.fsi.2023.109002. Epub 2023 Aug 15.

Authors

Meiqi Dai¹, Yuting Zhang¹, Yu Jiao², Yuewen Deng³, Xiaodong Du⁴, Chuangye Yang⁵

Affiliations

¹ Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China.
² Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang, 524088, China. Electronic address: jiaoy@gdou.edu.cn.
³ Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang, 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, China; Guangdong Marine Ecology Early Warning and Monitoring Laboratory, Zhanjiang, 524088, China.
⁴ Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang, 524088, China.
⁵ Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; Guangdong Marine Ecology Early Warning and Monitoring Laboratory, Zhanjiang, 524088, China.

PMID: 37586600
DOI: 10.1016/j.fsi.2023.109002

Abstract

Novel microRNA miR-63 (novel-miR-63) from pearl oyster Pinctada fucata martensii (Pm-novel-miR-63) is a species-specific miRNA. Our previous research has shown that the expression of Pm-novel-miR-63 was significantly downregulated at 24 h after nucleus transplantation. In this study, we analyzed the function and regulatory role of Pm-novel-miR-63 in the immune response of pearl oysters. The results showed that Pm-novel-miR-63 expression increased after the stimulation of pathogen associated molecular patterns at 6-12 h, and the activity of immune and antioxidant enzymes in the serum decreased after Pm-novel-miR-63 overexpression. Transcriptome analysis revealed that Pm-novel-miR-63 participated in regulating transplantation immunity through the Notch and mRNA surveillance signaling pathways. Target prediction and dual luciferase analysis revealed that Pm-GDP-FucTP, Pm-CysLTR2, and Pm-RLR were the target genes of Pm-novel-miR-63. These results suggested that Pm-novel-miR-63 participated in regulating the immune response in pearl oysters and can serve as a new interference target to reasonably control excessive immune rejection in pearl culture.

Keywords: Pinctada fucata martensii; Pm-novel-miR-63; Transplant immunization.

MeSH terms

Animals
Antioxidants / metabolism
Gene Expression Profiling / veterinary
Immunity
MicroRNAs* / metabolism
Pinctada*

Substances

MicroRNAs
Antioxidants