Development of novel nanocomposite-modified photoelectrochemical sensor based on the association of bismuth vanadate and MWCNT-grafted-molecularly imprinted poly(acrylic acid) for dopamine determination at nanomolar level

Felipe Augusto Gorla; Caroline Santana Dos Santos; Roberto de Matos; Roberta Antigo Medeiros; Milena do Prado Ferreira; Luan Pereira Camargo; Luiz Henrique Dall'Antonia; César Ricardo Teixeira Tarley

doi:10.1016/j.talanta.2023.125044

Development of novel nanocomposite-modified photoelectrochemical sensor based on the association of bismuth vanadate and MWCNT-grafted-molecularly imprinted poly(acrylic acid) for dopamine determination at nanomolar level

Talanta. 2024 Jan 1;266(Pt 2):125044. doi: 10.1016/j.talanta.2023.125044. Epub 2023 Aug 7.

Authors

Felipe Augusto Gorla¹, Caroline Santana Dos Santos², Roberto de Matos², Roberta Antigo Medeiros², Milena do Prado Ferreira², Luan Pereira Camargo², Luiz Henrique Dall'Antonia³, César Ricardo Teixeira Tarley⁴

Affiliations

¹ Federal Institute of Parana (IFPR), Campus Assis Chateaubriand, Avenida Cívica 475, Centro Cívico, Assis Chateaubriand, Parana, 85935-000, Brazil; State University of Londrina (UEL), Department of Chemistry, Rodovia Celso Garcia Cid, PR 445 Km 380, Londrina, Parana, 86050-482, Brazil.
² State University of Londrina (UEL), Department of Chemistry, Rodovia Celso Garcia Cid, PR 445 Km 380, Londrina, Parana, 86050-482, Brazil.
³ State University of Londrina (UEL), Department of Chemistry, Rodovia Celso Garcia Cid, PR 445 Km 380, Londrina, Parana, 86050-482, Brazil; National Institute of Science and Technology in Bioanalysis (INCTBio), State University of Campinas (UNICAMP), Department of Analytical Chemistry, Cidade Universitária Vaz S/n, Campinas, Sao Paulo 13083-970, Brazil.
⁴ State University of Londrina (UEL), Department of Chemistry, Rodovia Celso Garcia Cid, PR 445 Km 380, Londrina, Parana, 86050-482, Brazil; National Institute of Science and Technology in Bioanalysis (INCTBio), State University of Campinas (UNICAMP), Department of Analytical Chemistry, Cidade Universitária Vaz S/n, Campinas, Sao Paulo 13083-970, Brazil. Electronic address: tarley@uel.br.

PMID: 37586281
DOI: 10.1016/j.talanta.2023.125044

Abstract

This study proposes the development of a new photoelectrochemical (PEC) sensor for the determination of dopamine (DA) at nanomolar levels. The PEC sensor was based on a physical mixture of bismuth vanadate (BiVO₄) with nanocomposite molecularly imprinted poly(acrylic acid) (MIP-AA) grafted onto MWCNT_ox by using the surface-controlled radical polymerization strategy with an INIFERTER reagent. XRD, diffuse reflectance spectroscopy (DRE), SEM, TEM, and TGA were employed to characterize the materials. Photoelectrochemical analyses were carried out with GCE/BiVO₄/MIP-AA sensor under visible light using a potential of 0.6 V, phosphate buffer (0.1 mol L^-1) at pH 7.0, and modifying the GCE with a film composed of monoclinic BiVO₄ at 3.5 mg mL^-1 and nanocomposite MIP prepared with acrylic acid (MIP-AA) at 0.1 mg mL^-1. The proposed method using the GCE/BiVO₄/MIP-AA sensor presented a limit of detection (LOD) of 2.9 nmol L^-1, a linear range from 9.7 to 150 nmol L^-1 and it was successfully applied for analysis of DA in urine samples using external calibration curve yielding recovery values of 90-105%. Additionally, the proposed PEC sensor allowed DA determination without interference from uric acid, ascorbic acid, epinephrine, norepinephrine, and other unwanted interferences.

Keywords: BiVO(4); Grafting from approach; Hydrothermal synthesis; Molecularly imprinted polymer; Synthetic urine.

MeSH terms

Dopamine / chemistry
Electrochemical Techniques / methods
Electrodes
Limit of Detection
Molecular Imprinting* / methods
Nanocomposites* / chemistry
Polymers / chemistry

Substances

Polymers
Dopamine
bismuth vanadium tetraoxide
carbopol 940