The presence of blastocyst within the uteri facilitates lumenal epithelium transformation for implantation via upregulating lysosome proteostasis activity

Autophagy. 2024 Jan;20(1):58-75. doi: 10.1080/15548627.2023.2247747. Epub 2023 Aug 22.

Abstract

ACTB: actin beta; AREG: amphiregulin; ATP6V0A4: ATPase, H+ transporting, lysosomal V0 subunit A4; Baf A1: bafilomycin A1; BSA: bovine serum albumin; CLDN1: claudin 1; CTSB: cathepsin B; DEGs: differentially expressed genes; E2: 17β-estradiol; ESR: estrogen receptor; GATA2: GATA binding protein 2; GLA: galactosidase, alpha; GO: gene ontology; HBEGF: heparin-binding EGF-like growth factor; IGF1R: insulin-like growth factor 1 receptor; Ihh: Indian hedgehog; ISH: in situ hybridization; LAMP1: lysosomal-associated membrane protein 1; LCM: laser capture microdissection; Le: lumenal epithelium; LGMN: legumain; LIF: leukemia inhibitory factor; LIFR: LIF receptor alpha; MSX1: msh homeobox 1; MUC1: mucin 1, transmembrane; P4: progesterone; PBS: phosphate-buffered saline; PCA: principal component analysis; PPT1: palmitoyl-protein thioesterase 1; PGR: progesterone receptor; PSP: pseudopregnancy; PTGS2/COX2: prostaglandin-endoperoxide synthase 2; qPCR: quantitative real-time polymerase chain reaction; SP: pregnancy; TFEB: transcription factor EB.

Keywords: Blastocyst; IGF2; embryo implantation; lysosome; uterine lumenal epithelium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autophagy
  • Blastocyst / metabolism
  • Cyclooxygenase 2 / metabolism
  • Epithelium / metabolism
  • Female
  • Hedgehog Proteins* / metabolism
  • Humans
  • Lysosomes / metabolism
  • Pregnancy
  • Proteostasis*
  • Uterus / metabolism

Substances

  • Hedgehog Proteins
  • Cyclooxygenase 2

Grants and funding

The work was supported by the National Key Research and Development Program of China [2022YFC2702500 and 2021YFC2700302]; National Natural Science Foundation of China (82288102, 81830045, 82030040, 82222026 and 81971388).