Superoxide dismutase ameliorates oxidative stress and regulates liver transcriptomics to provide therapeutic benefits in hepatic inflammation

Longyan Chen; Yang Liu; Yonggang Zhang; Yanmin Zhang; Wei Wang; Hongyu Han; Chunyu Yang; Xueqian Dong

doi:10.7717/peerj.15829

Superoxide dismutase ameliorates oxidative stress and regulates liver transcriptomics to provide therapeutic benefits in hepatic inflammation

PeerJ. 2023 Aug 11:11:e15829. doi: 10.7717/peerj.15829. eCollection 2023.

Authors

Longyan Chen¹, Yang Liu², Yonggang Zhang², Yanmin Zhang², Wei Wang², Hongyu Han², Chunyu Yang³, Xueqian Dong^{2

3}

Affiliations

¹ Qilu Hospital of Shandong University, Jinan, China.
² QiLu University of Technology, Jinan, China.
³ State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Abstract

Background: Oxidative stress refers to the imbalance between oxidants and antioxidants in organisms and often induces hepatic inflammation. Supplementing exogenous superoxide dismutase is an effective way to alleviate oxidative stress; however, the effects and mechanisms by which superoxide dismutase alleviates hepatic inflammation remain unclear.

Methods: This study established a Kunming mouse model to verify and investigate the oxidative stress and hepatic inflammation-alleviating effects of the superoxide dismutase oral supplement that was prepared by our research group in a previous study.

Results: The superoxide dismutase product significantly restored the body weight and liver alanine transaminase, aspartate aminotransferase, superoxide dismutase, catalase, glutathione, and glutathione peroxidase levels of oxidative stress induced mice. Moreover, exogenous superoxide dismutase significantly inhibited interleukin 1β and interleukin 6 mRNA expression in the livers of mice with hepatic inflammation. Transcriptomic analysis indicated that superoxide dismutase had a significant inhibitory effect on Endog expression, alleviating oxidative stress damage, and mediating liver cell apoptosis by regulating the expression of Rab5if, Hnrnpab, and Ifit1.

Conclusion: Our research verified the oxidative stress remediation effects of superoxide dismutase and its therapeutic role against hepatic inflammation. This study can lay a foundation for investigating the mechanism by which superoxide dismutase alleviates hepatic disease.

Keywords: Deoxynivalenol; Hepatic inflammation; Oxidative stress; Superoxide dismutase; Transcriptomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Inflammation / drug therapy
Liver*
Mice
Oxidative Stress
Superoxide Dismutase / metabolism
Transcriptome*

Substances

Superoxide Dismutase

Grants and funding

This work was supported by the National Key Research and Development program of China under Grant [2021YFC2103204]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.