Analysis of Protein Degradation in Ferroptosis

Zhuojun Zhang; Lili Jiang

doi:10.1007/978-1-0716-3433-2_12

Analysis of Protein Degradation in Ferroptosis

Methods Mol Biol. 2023:2712:135-148. doi: 10.1007/978-1-0716-3433-2_12.

Authors

Zhuojun Zhang^{1

2}, Lili Jiang^{3

4}

Affiliations

¹ Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, China.
² Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, China.
³ Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, China. jianglili@gzhmu.edu.cn.
⁴ Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, China. jianglili@gzhmu.edu.cn.

PMID: 37578702
DOI: 10.1007/978-1-0716-3433-2_12

Abstract

The ubiquitin-proteasome system (UPS) is a highly conserved cellular mechanism that degrades and recycles proteins in eukaryotic cells. It involves the tagging of specific target proteins with ubiquitin, a small regulatory protein, which marks them for degradation by the proteasome, a large protein complex that acts as a molecular shredder. Dysfunction of the UPS has been implicated in a wide range of diseases, including cancer, neurodegenerative disorders, and viral infections. Therefore, targeting the UPS has become an attractive therapeutic strategy for many diseases. Ferroptosis is an iron-dependent cell death process that is regulated by multiple levels, including protein degradation. In this chapter, we introduce the detection of UPS-mediated protein degradation in ferroptosis using several techniques such as western blotting, co-immunoprecipitation, in vitro ubiquitination assay, and proteasome assay.

Keywords: Ferroptosis; Protein degradation; Ubiquitin-proteasome system.