Organoids: opportunities and challenges of cancer therapy

Xianjie Jiang; Linda Oyang; Qiu Peng; Qiang Liu; Xuemeng Xu; Nayiyuan Wu; Shiming Tan; Wenjuan Yang; Yaqian Han; Jinguan Lin; Longzheng Xia; Mingjing Peng; Yanyan Tang; Xia Luo; Min Su; Yingrui Shi; Yujuan Zhou; Qianjin Liao

doi:10.3389/fcell.2023.1232528

Organoids: opportunities and challenges of cancer therapy

Front Cell Dev Biol. 2023 Jul 27:11:1232528. doi: 10.3389/fcell.2023.1232528. eCollection 2023.

Authors

Xianjie Jiang^{1

2}, Linda Oyang^{1

2}, Qiu Peng^{1

2}, Qiang Liu^{1

2}, Xuemeng Xu^{1

3}, Nayiyuan Wu^{1

2}, Shiming Tan¹, Wenjuan Yang¹, Yaqian Han^{1

2}, Jinguan Lin¹, Longzheng Xia¹, Mingjing Peng^{1

2}, Yanyan Tang^{1

2}, Xia Luo^{1

2}, Min Su^{1

2}, Yingrui Shi^{1

2}, Yujuan Zhou^{1

2}, Qianjin Liao^{1

2}

Affiliations

¹ Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital, Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
² Public Service Platform of Tumor Organoids Technology, Changsha, Hunan, China.
³ Hengyang Medical School, University of South China, Hengyang, Hunan, China.

Abstract

Organoids are a class of multicellular structures with the capability of self-organizing and the characteristic of original tissues, they are generated from stem cells in 3D culture in vitro. Organoids can mimic the occurrence and progression of original tissues and widely used in disease models in recent years. The ability of tumor organoids to retain characteristic of original tumors make them unique for tumorigenesis and cancer therapy. However, the history of organoid development and the application of organoid technology in cancer therapy are not well understood. In this paper, we reviewed the history of organoids development, the culture methods of tumor organoids establishing and the applications of organoids in cancer research for better understanding the process of tumor development and providing better strategies for cancer therapy. The standardization of organoids cultivation facilitated the large-scale production of tumor organoids. Moreover, it was found that combination of tumor organoids and other cells such as immune cells, fibroblasts and nervous cells would better mimic the microenvironment of tumor progression. This might be important developing directions for tumor organoids in the future.

Keywords: application; cancer therapy; establishing; organoids; organoids development.

Publication types

Review

Grants and funding

This work was supported in part by grants from the following sources: the National Natural Science Foundation of China (82203233, 82202966, 82173142, 81972636, 81872281), the Natural Science Foundation of Hunan Province (2022JJ80078, 2020JJ5336), the Research Project of Health Commission of Hunan Province (202203034978, 202109031837, 20201020), Key Research and Development Program of Hunan Province (2022SK 2051), Hunan Provincial Science and Technology Department (2020TP1018), the Changsha Science and Technology Board (kh2201054), Ascend Foundation of National cancer center (NCC201909B06), Hunan Cancer Hospital Climb Plan (ZX2020001-3, YF2020002), and by China Postdoctoral Science Foundation (2022TQ0104, 2022M721118).