Metagenomic characterization of the enhanced performance of multicomponent synergistic thermophilic anaerobic co-digestion of food waste utilizing kitchen waste or garden waste as co-substrate

Siying Zhang; Chengyu Liang; Mengyao Xiao; Chunmeng Chui; Na Wang; Yuji Ji; Zhi Wang; Jiping Shi; Li Liu

doi:10.1016/j.watres.2023.120457

Metagenomic characterization of the enhanced performance of multicomponent synergistic thermophilic anaerobic co-digestion of food waste utilizing kitchen waste or garden waste as co-substrate

Water Res. 2023 Oct 1:244:120457. doi: 10.1016/j.watres.2023.120457. Epub 2023 Aug 7.

Authors

Siying Zhang¹, Chengyu Liang², Mengyao Xiao¹, Chunmeng Chui³, Na Wang¹, Yuji Ji³, Zhi Wang³, Jiping Shi⁴, Li Liu⁵

Affiliations

¹ Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China; University of Chinese Academy of Sciences, Beijing 100049, China.
² State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
³ Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
⁴ Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China; School of Life Science and Technology, Shanghai Tech University, Shanghai 201210, China. Electronic address: shijp@sari.ac.cn.
⁵ Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China; Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, Shanghai 200241, China. Electronic address: liul@sari.ac.cn.

PMID: 37574624
DOI: 10.1016/j.watres.2023.120457

Abstract

Food waste (FW) single-substrate anaerobic digestion usually suffers from rapid acidification and inhibition of oil and salt. To overcome these problems and improve the process efficiency, supplementing other substrates has been used in FW anaerobic digestion. This study investigated the biogas production potential through co-digestion of FW with kitchen waste (KW) or garden waste (GW) in different ratios under thermophilic conditions. The results showed that the optimal ratios were FW:KW=60:40 and FW:GW=80:20 which biogas production improved 73.33% and 68.45% compared with single FW digestion, respectively. The organic matter removal rate of co-digestion was 84.46% for FW+KW group (R_FK) and 65.64% for FW+GW group (R_FG). Co-digestion increased the abundance of the dominant hydrolytic bacteria Defluviitoga and Hydrogenispora and hydrogenotrophic methanogen Methanoculleus. Furthermore, glycoside hydrolases (GHs), vital carbohydrate-active enzymes (CAZymes), were improved by co-digestion. Co-digestion could also effectively promote the function of cellulase and hemicellulose. This strategy for utilizing different organic wastes together as co-substrate provides a new avenue for bioenergy production.

Keywords: Carbohydrate-active enzymes; Food waste; Garden waste; Kitchen waste; Metabolism pathways; Thermophilic anaerobic co-digestion.

MeSH terms

Anaerobiosis
Biofuels
Bioreactors
Digestion
Food
Gardens
Methane
Refuse Disposal* / methods
Sewage

Substances

Biofuels
Methane
Sewage